1. 重组蛋白
  2. Enzymes & Regulators
  3. Serine/Threonine Kinase Proteins
  4. Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1/IRE1)
  5. IRE1 蛋白, Human (sf9)

IRE1 蛋白, Human (sf9)

目录号: HY-P73858
COA 产品使用指南

IRE1 是一种关键的丝氨酸/苏氨酸蛋白激酶和内切核糖核酸酶,对于感测内质网中的未折叠蛋白反应 (UPR) 至关重要。在细胞稳态中,它通过与 HSPA5/BiP 结合而保持单体状态。IRE1 蛋白, Human (sf9) 是重组的 IRE1 蛋白,由 Sf9 insect cells 表达,不带标签。IRE1 蛋白, Human (sf9) 全长 513 个氨基酸,分子量约为 ~65 kDa。

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描述

IRE1 is a key serine/threonine protein kinase and endoribonuclease essential for sensing the unfolded protein response (UPR) in the endoplasmic reticulum. In cellular homeostasis, it remains monomeric by binding to HSPA5/BiP. IRE1 Protein, Human (sf9) is the recombinant human-derived IRE1 protein, expressed by Sf9 insect cells , with tag free. The total length of IRE1 Protein, Human (sf9) is 513 a.a., with molecular weight of ~65 kDa.

研究背景

IRE1 是一种丝氨酸/苏氨酸蛋白激酶和内切核糖核酸酶,是内质网未折叠蛋白反应 (UPR) 中的关键传感器。在没有细胞应激的情况下,内质网管腔结构域通过与内质网伴侣 HSPA5/BiP 结合来维持其非活性单体状态。当错误折叠蛋白积累时,HSPA5/BiP 的释放使管腔结构域同二聚化,促进激酶结构域的自磷酸化以及随后核糖核酸内切酶活性的激活。这种核糖核酸内切酶活性对 XBP1 mRNA 具有特异性,可从 XBP1 转录物中切除 26 个核苷酸。由此产生的剪接 XBP1 转录本编码一种转录激活因子,可上调 UPR 靶基因的表达。除了在 UPR 中的作用之外,IRE1 还通过调节 SEC16A 的表达和定位,作为 ER 应激诱导的 GORASP2 介导的 CFTR 非常规转运至细胞膜的上游信号,强调了它参与 UPR 之外的多种细胞过程。

生物活性

Measured by its nuclease activity to cleave Xbp1 single stem-loop mini-substrate.

种属

Human

表达系统

Sf9 insect cells

标签

Tag Free

蛋白编号

O75460 (P465-L977)

基因 ID
蛋白结构
N-term
IRE1 (P465-L977)
Accession # O75460
C-term
同用名
Serine/threonine-protein kinase/endoribonuclease IRE1; IRE1a; ERN1; IRE1
分子量

Approximately 65 kDa

纯度

Greater than 80% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IRE1 Protein, Human (sf9)
目录号:
HY-P73858
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