JAML/AMICA 蛋白, Mouse (HEK293, Fc)

JAML/AMICA proteins are predicted to have integrin-binding and homodimerization activities, regulate gamma-delta T cell activation and promote epithelial cell proliferation in wound healing. It mainly exists in the plasma membrane, is an ortholog of human JAML, and is involved in cell adhesion. JAML/AMICA Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived JAML/AMICA protein, expressed by HEK293 , with C-hFc labeled tag. The total length of JAML/AMICA Protein, Mouse (HEK293, Fc) is 261 a.a., with molecular weight of ~56.5 KDa.

JAML/AMICA 蛋白预计具有整合素结合活性和蛋白同二聚化活性。它在 γ-δ T 细胞激活中发挥作用，并积极调节上皮细胞增殖，特别是在与伤口愈合相关的过程中。该蛋白质主要位于质膜中。作为人类 JAML（类似连接粘附分子）的直系同源物，JAML/AMICA 参与细胞粘附过程。尽管其功能重要，但在参考数据集中观察到低表达水平，表明其调节功能可能受到严格控制或依赖于上下文。该基因与细胞粘附过程的关联及其低表达水平突显了其在特定生理环境中的潜在参与，特别是与免疫反应和组织修复相关的生理环境。

Measured by the ability of the immobilized protein to support the adhesion of the A549 Human non-small cell lung cancer cells. The adhesion rate for this effect is 55.10 % in the presence of 10 μg/mL JAML.

Mouse

HEK293

C-hFc

NP_001005421.3 (Q21-L281)

270152  [NCBI]

QGLPGLTVSSPQLRVHVGESVLMGCVVQRTEEKHVDRVDWLFSKDKDDASEYVLFYYSNLSVPTGRFQNRSHLVGDTFHNDGSLLLQDVQKADEGIYTCEIRLKNESMVMKKPVELWVLPEEPKDLRVRVGDTTQMRCSIQSTEEKRVTKVNWMFSSGSHTEEETVLSYDSNMRSGKFQSLGRFRNRVDLTGDISRNDGSIKLQTVKESDQGIYTCSIYVGKLESRKTIVLHVVQDEFQRTISPTPPTDKGQQGILNGNQL

Approximately 56.5 kDa.

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.