Kallikrein-11 蛋白, Human (HEK293, His)

Kallikrein-11 protein is suggested to be a multifunctional protease, efficiently cleaving the substrate 'bz-Phe-Arg-4-methylcoumaryl-7-amide' and showing weak cleavage activity toward other kallikrein and trypsin substrates. It predominantly cleaves synthetic peptides after arginine residues rather than lysine residues. Kallikrein-11 Protein, Human (HEK293, His) is the recombinant human-derived Kallikrein-11 protein, expressed by HEK293 , with C-His labeled tag. The total length of Kallikrein-11 Protein, Human (HEK293, His) is 232 a.a., with molecular weight of ~40 kDa.

Kallikrein-11 蛋白被推测是一种潜在的多功能蛋白酶。它已被证明可以有效裂解称为“bz-Phe-Arg-4-methylcoumaryl-7-amide”的激肽释放酶底物，并对激肽释放酶和胰蛋白酶的其他底物表现出弱裂解活性。值得注意的是，它主要在精氨酸残基而不是赖氨酸残基之后切割合成肽。

Measured by its ability to cleave a colorimetric peptide substrate D-Val-Leu-Lys-ThioBenzyl ester (VLK-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB) (Edwards, K.M. et al.,1999, J. Biol. Chem. 274: 30468) . The specific activity is >200 pmol/min/μg.
(Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)

Human

HEK293

C-His

Q9UBX7 (E19-N250)

11012  [NCBI]

Approximately 40 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.