Kallikrein-2 蛋白, Human (HEK293, His)

The Kallikrein-2 protein is a member of the glandular kallikrein family and is critical in the enzymatic process as it selectively cleaves the Met-Lys and Arg-Ser bonds in kininogen, thereby releasing Lys-bradykinin . This proteolytic activity contributes to the production of bradykinin, a bioactive peptide with multiple roles in regulating blood pressure, inflammation, and vascular permeability. Kallikrein-2 Protein, Human (HEK293, His) is the recombinant human-derived Kallikrein-2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Kallikrein-2 Protein, Human (HEK293, His) is 243 a.a., with molecular weight of ~32.0 kDa.

Kallikrein-2 是腺激肽释放酶家族的成员，通过裂解激肽原中的 Met-Lys 和 Arg-Ser 键表现出酶活性，导致 Lys-缓激肽的释放。作为激肽-激肽释放酶系统的一部分，激肽释放酶-2 有助于缓激肽的生成，缓激肽是一种有效的血管活性肽，参与血压、炎症和血管通透性的调节。通过特异性裂解激肽原中的这些键，Kallikrein-2 在赖氨酸缓激肽的受控释放中发挥着至关重要的作用，从而影响下游的生理过程。

Measured by its ability to cleave a flourogenic peptide substrate Z-Phe-Arg-7-amido-4-methylcoumarin (FR-AMC). The specific activity is 198 pmol/min/μg.

Human

HEK293

C-6*His

P20151 (P19-P261)

3817  [NCBI]

PLIQSRIVGGWECEKHSQPWQVAVYSHGWAHCGGVLVHPQWVLTAAHCLKKNSQVWLGRHNLFEPEDTGQRVPVSHSFPHPLYNMSLLKHQSLRPDEDSSHDLMLLRLSEPAKITDVVKVLGLPTQEPALGTTCYASGWGSIEPEEFLRPRSLQCVSLHLLSNDMCARAYSEKVTEFMLCAGLWTGGKDTCGGDSGGPLVCNGVLQGITSWGPEPCALPEKPAVYTKVVHYRKWIKDTIAANP

Approximately 32.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Acetate, 250 mM Trehalose, 0.02% Tween 80, pH 5.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.