Kallikrein-8 蛋白, Human (260a.a, HEK293, His)

Kallikrein-8 protein is a serine protease that degrades proteins such as casein, fibrinogen, kininogen, fibronectin, and type IV collagen. It cleaves L1CAM in response to neural activity, promoting neurite growth in hippocampal neurons. Kallikrein-8 Protein, Human (260a.a, HEK293, His) is the recombinant human-derived Kallikrein-8 protein, expressed by HEK293 , with C-His labeled tag. The total length of Kallikrein-8 Protein, Human (260a.a, HEK293, His) is 260 a.a., with molecular weight of ~36 kDa.

Kallikrein-8 蛋白是一种丝氨酸蛋白酶，以其降解各种蛋白质的能力而闻名，包括酪蛋白、纤维蛋白原、激肽原、纤连蛋白和 IV 型胶原蛋白。它还会响应神经活动的增强而裂解 L1CAM，导致培养的海马神经元中的神经突生长和束颤。该蛋白在谢弗侧支通路中孤儿和小突触纽带的形成和成熟中起着至关重要的作用，从而调节海马体中谢弗侧支的长期增强，并有助于记忆获取和突触可塑性。此外，Kallikrein-8 参与皮肤脱屑、角质形成细胞增殖，并在脊髓损伤后发病机制的第二阶段发挥作用。

Measured by its ability to cleave the fluorogenic peptide substrate BocVPRAMC. The specific activity is > 400pmol/min/μg

Human

HEK293

C-His

O60259 (M1-G260)

11202  [NCBI]

Approximately 36 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.