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  3. Ki67/MKI67 蛋白, Human (GST)

Ki67/MKI67 蛋白, Human (GST)

目录号: HY-P72508
COA 产品使用指南

Ki67/MKI67 位于有丝分裂染色体上,并在核膜解体过程中保持其分散性。它覆盖了染色体表面的很大一部分,并防止染色质塌陷成团。Ki67/MKI67 蛋白, Human (GST) 是重组的 Ki67/MKI67 蛋白,由 E. coli 表达,带有 N-GST 标签。Ki67/MKI67 蛋白, Human (GST) 全长 120 个氨基酸,分子量约为 approximately 36.91 kDa。

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10 μg ¥950 In-stock
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1 mg ¥15120 In-stock
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描述

Ki67/MKI67 is localized on mitotic chromosomes and maintains its dispersion during nuclear envelope disassembly. It covers a large portion of the chromosome surface and prevents chromatin from collapsing into clumps. Ki67/MKI67 Protein, Human (GST) is the recombinant human-derived Ki67/MKI67 protein, expressed by E. coli , with N-GST labeled tag. The total length of Ki67/MKI67 Protein, Human (GST) is 120 a.a., with molecular weight of approximately 36.91 kDa.

研究背景

Ki67/MKI67 蛋白对于维持核膜解体后单个有丝分裂染色体在细胞质中的分散至关重要。Ki67/MKI67 位于有丝分裂染色体的表面,特别是在染色体周围层内,覆盖了染色体表面的很大一部分,防止染色体塌陷成单个染色质团。作为具有高净电荷的表面活性剂,它建立了空间和静电荷屏障,促进独立的染色体运动。Ki67/MKI67 表现出 DNA 结合能力,显示出对超螺旋 DNA 和富含 AT 的 DNA 的偏好。虽然它对有丝分裂染色体的内部结构没有贡献,但它在染色质组织中的作用仍然不确定,这增加了这可能是其维持分散的有丝分裂染色体的主要功能的间接结果的可能性。该蛋白与各种伙伴相互作用,包括 KIF15、NIFK、PPP1CC,并形成涉及 ZNF335、HCFC1、CCAR2、EMSY、RBBP5、ASH2L 和 WDR5 的复合物的一部分,表明其参与复杂的细胞过程和分子网络。

种属

Human

表达系统

E. coli

标签

N-GST

蛋白编号

P46013 (M1-P120)

基因 ID
蛋白结构
N-term
GST
Ki67 (M1-P120)
Accession # P46013
C-term
同用名
Antigen KI-67; Ki-67; KIA; MIB-1; MKI67; Proliferation Marker Protein Ki-67
氨基酸序列

MWPTRRLVTIKRSGVDGPHFPLSLSTCLFGRGIECDIRIQLPVVSKQHCKIEIHEQEAILHNFSSTNPTQVNGSVIDEPVRLKHGDVITIIDRSFRYENESLQNGRKSTEFPRKIREQEP

分子量

approximately 36.91 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Ki67/MKI67 Protein, Human (GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Ki67/MKI67 Protein, Human (GST)
目录号:
HY-P72508
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