KREMEN1 蛋白, Human (HEK293, His)

KREMEN1 Protein, Human (HEK293, His) is the recombinant human-derived KREMEN1 protein, expressed by HEK293, with C-His labeled tag.

KREMEN1 是一种 Dickkopf 蛋白的受体，通过与 DKK1/2 协同作用抑制 Wnt/beta-catenin 信号通路，促进 Wnt 受体 LRP5 和 LRP6 的内吞。在缺乏 DKK1 的情况下，KREMEN1 通过维持 LRP5 或 LRP6 在细胞膜上的存在增强 Wnt-beta-catenin 信号传导。此外，KREMEN1 能够以不依赖 Wnt 的方式触发细胞凋亡，而这种凋亡活性在结合配体 DKK1 时受到抑制。在肢体发育中，KREMEN1 通过减弱 Wnt 信号传导促进正常的肢体模式形成，并负调控骨形成。在耳蜗发育过程中，KREMEN1 调节细胞命运决定，对毛细胞命运特化具有抑制作用。

Measured by its binding ability in a functional ELISA. Immobilized human Dkk-1 at 2 μg/mL can bind Human KREMEN1. The ED₅₀ for this effect is 0.315 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized human Dkk-1 at 2 μg/mL can bind Human KREMEN1. The ED₅₀ for this effect is 0.315 μg/mL.

Human

HEK293

C-His

Q96MU8-2/NP_114434.3 (A20-T394)

83999  [NCBI]

Partial

ARPAPSPGLGPGPECFTANGADYRGTQNWTALQGGKPCLFWNETFQHPYNTLKYPNGEGGLGEHNYCRNPDGDVSPWCYVAEHEDGVYWKYCEIPACQMPGNLGCYKDHGNPPPLTGTSKTSNKLTIQTCISFCRSQRFKFAGMESGYACFCGNNPDYWKYGEAASTECNSVCFGDHTQPCGGDGRIILFDTLVGACGGNYSAMSSVVYSPDFPDTYATGRVCYWTIRVPGASHIHFSFPLFDIRDSADMVELLDGYTHRVLARFHGRSRPPLSFNVSLDFVILYFFSDRINQAQGFAVLYQAVKEELPQERPAVNQTVAEVITEQANLSVSAARSSKVLYVITTSPSHPPQTVPGSNSWAPPMGAGSHRVEGWT

Approximately 60 kDa. The protein migrates as an approximately 60 kDa band under reducing SDS-PAGE due to glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.