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  4. LacI 蛋白, E.coli (His-SUMO)

LacI 蛋白, E.coli (His-SUMO)

目录号: HY-P71519
产品使用指南

LacI 是乳糖操纵子阻遏蛋白，通过与操纵子区域结合并抑制基因转录来调节乳糖代谢。诱导剂异乳糖的存在会改变其结合亲和力，从而解除操纵子的抑制。作为同源四聚体，LacI 可以精细调节乳糖代谢，协调调节机制以响应细胞环境。LacI 蛋白, E.coli (His-SUMO) 是重组的 LacI 蛋白，由 E. coli 表达，带有 N-His, N-SUMO 标签。LacI 蛋白, E.coli (His-SUMO) 全长 360 个氨基酸，分子量约为 ~54.6 kDa。

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描述

LacI, the lactose operon repressor, regulates lactose metabolism by binding to the operator region and repressing gene transcription. The presence of the inducer, allolactose, alters its binding affinity, derepressing the operon. As a homotetramer, LacI finely tunes lactose metabolism, orchestrating regulatory mechanisms in response to the cellular environment. LacI Protein, E.coli (His-SUMO) is the recombinant E. coli-derived LacI protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of LacI Protein, E.coli (His-SUMO) is 360 a.a., with molecular weight of ~54.6 kDa.
研究背景

LacI 是乳糖操纵子的阻遏蛋白，在乳糖代谢的调节中起着至关重要的作用。它通过与操纵基因区域结合并抑制乳糖利用相关基因的转录来发挥作用。诱导剂异乳糖的存在会改变结合亲和力，从而导致操纵子的去抑制。作为同四聚体，LacI 协调调节机制，根据细胞环境微调乳糖代谢。
种属

E.coli
表达系统

E. coli
标签

N-His;N-SUMO
蛋白编号

P03023 (1M-360Q)
基因 ID

945007  [NCBI]
蛋白结构
N-term
6*His-SUMO
LacI (1M-360Q)
Accession # P03023
C-term
同用名
lacI; b0345; JW0336; Lactose operon repressor
氨基酸序列

MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ

分子量

Approximately 54.6 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

LacI Protein, E.coli (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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