LAMP1/CD107a 蛋白, Rat (HEK293, Fc)

The LAMP1/CD107a protein is a lysosomal membrane glycoprotein that plays critical roles in lysosomal biogenesis, pH regulation, autophagy, and cholesterol homeostasis. It critically regulates lysosomal lumen pH by inhibiting the proton channel TMEM175, optimizing lysosomal acidification for efficient hydrolase activity. LAMP1/CD107a Protein, Rat (HEK293, Fc) is the recombinant rat-derived LAMP1/CD107a protein, expressed by HEK293 , with C-hFc labeled tag. The total length of LAMP1/CD107a Protein, Rat (HEK293, Fc) is 350 a.a., with molecular weight of ~125 kDa.

LAMP1/CD107a 蛋白是一种溶酶体膜糖蛋白，在溶酶体生物合成、溶酶体 pH 调节、自噬和胆固醇稳态中发挥着关键作用。它通过直接抑制质子通道 TMEM175 充当溶酶体腔 pH 值的重要调节剂，促进最佳溶酶体酸化以实现有效的水解酶活性。除了参与细胞稳态之外，LAMP1 对 NK 细胞的细胞毒性也有显着贡献。从机制上讲，它参与细胞毒性颗粒向细胞表面的移动以及穿孔素向裂解颗粒的运输，保护 NK 细胞免受其自身细胞毒性颗粒含量引起的脱颗粒相关损伤。此外，LAMP1 在将碳水化合物配体呈递给选择素方面发挥作用。与 ABCB9 和 FURIN 等蛋白质的相互作用至关重要，可以稳定 ABCB9 并保护其免遭溶酶体降解，同时还抑制 TMEM175 的质子通道活性。

Rat

HEK293

C-hFc

P14562 (A22-N371)

25328  [NCBI]

APALFEVKDNNGTACIMASFSASFLTTYDAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTTRYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTIVLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGTCLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFGMNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKALALNVFSVQVQAFRVESDRFGSVEECVQDGNN

Approximately 125 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.