LDCC 蛋白, E.coli (His)

LDCC protein is also called lysine decarboxylase. As an enzyme that can catalyze the decarboxylation of lysine, it plays a key role in the utilization of lysine. This enzymatic activity enables LDCC to remove carboxyl groups from lysine, thereby generating cadaverine, a biogenic amine. LDCC Protein, E.coli (His) is the recombinant E. coli-derived LDCC protein, expressed by E. coli , with C-6*His labeled tag. The total length of LDCC Protein, E.coli (His) is 713 a.a., with molecular weight of 85 kDa.

LDCC蛋白也称为赖氨酸脱羧酶，作为能够催化赖氨酸脱羧的酶，在赖氨酸的利用中发挥着关键作用。这种酶活性使 LDCC 能够去除赖氨酸中的羧基，从而生成尸胺（一种生物胺）。尸胺参与多种生物过程，并参与细胞生长、分化和免疫反应的调节。LDCC 作为赖氨酸脱羧酶的功能对于细胞代谢中赖氨酸的有效利用至关重要，需要进一步研究以充分了解其调节机制和在各种生物环境中的潜在应用。

1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Measured by its binding ability in a functional ELISA. Immobilized ldcC at 2 μg/mL can bind human ycbX, the EC₅₀ of human ycbX protein is ≤90 μg/mL.

E.coli

E. coli

C-6*His

P52095 (M1-G713)

66671526  [NCBI]

MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLKFIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWFFEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQKSPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTNGTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIPRREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWVPYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEAFMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWFFDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEGIPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRIKNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAWQRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSVGQHYPGFETDIHGAKQDEDGVYRVRVLKMAG

85 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.