2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. Leukotriene A4 Hydrolase/LTA4H 蛋白, Mouse (sf9, His)

Leukotriene A4 Hydrolase/LTA4H 蛋白, Mouse (sf9, His)

目录号: HY-P74776
产品使用指南

LTA4H 蛋白是一种双功能锌金属酶，充当环氧化物水解酶，将白三烯 A4 (LTA4) 转化为促炎白三烯 B4 (LTB4)。它还充当氨肽酶，裂解 N 末端精氨酸并对抗炎症，特别是通过 Pro-Gly-Pro (PGP) 裂解。LTA4H 有助于 resolvin E1 和 18S-resolvin E1 生物合成，产生有效的抗炎和促溶解脂质介质。其多方面的活性强调了其在调节炎症过程和脂质介质生物合成中的复杂作用。Leukotriene A4 Hydrolase/LTA4H 蛋白, Mouse (sf9, His) 是重组的 白三烯 A4 水解酶/LTA4H 蛋白，由 Sf9 昆虫细胞表达，带有 C-His 标签。Leukotriene A4 Hydrolase/LTA4H 蛋白, Mouse (sf9, His) 全长 611 个氨基酸，分子量约为 ~62 kDa。

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描述

LTA4H Protein, a bifunctional zinc metalloenzyme, functions as an epoxide hydrolase, converting leukotriene A4 (LTA4) to pro-inflammatory leukotriene B4 (LTB4). It also acts as an aminopeptidase, cleaving N-terminal arginines and countering inflammation, particularly through Pro-Gly-Pro (PGP) cleavage. LTA4H contributes to resolvin E1 and 18S-resolvin E1 biosynthesis, generating potent anti-inflammatory and pro-resolving lipid mediators. Its multifaceted activities underscore its intricate role in regulating inflammatory processes and lipid mediator biosynthesis. Leukotriene A4 Hydrolase/LTA4H Protein, Mouse (sf9, His) is the recombinant mouse-derived Leukotriene A4 Hydrolase/LTA4H protein, expressed by Sf9 insect cells, with C-His labeled tag. The total length of Leukotriene A4 Hydrolase/LTA4H Protein, Mouse (sf9, His) is 611 a.a., with molecular weight of ~62 kDa.
研究背景

白三烯 A4 水解酶/LTA4H 蛋白是一种双功能锌金属酶，具有环氧化物水解酶 (EH) 和氨肽酶活性。作为环氧化物水解酶，它催化白三烯 A4 (LTA4) 转化为促炎介质白三烯 B4 (LTB4)。此外，LTA4H 具有氨肽酶活性，对各种合成三肽中的 N 末端精氨酸具有高亲和力。除了其促炎 EH 作用外，该蛋白还可以通过其氨肽酶活性来对抗炎症，氨肽酶活性会裂解中性粒细胞吸引剂三肽 Pro-Gly-Pro (PGP)——一种由基质金属蛋白酶 9 (MMP9) 和脯氨酰肽酶 (PREPL) 产生的生物活性胶原蛋白片段。）。此外，LTA4H 参与二十碳五烯酸生物合成 resolvin E1 和 18S-resolvin E1，有助于产生具有强效抗炎和促溶解作用的脂质介质。LTA4H 的多方面活性凸显了其在炎症过程和脂质介质生物合成调节中的复杂参与。
种属

Mouse
表达系统

Sf9 insect cells
标签

C-His
蛋白编号

P24527 (M1-D611)
基因 ID

16993  [NCBI]
蛋白结构
N-term
LTA4H (M1-D611)
Accession # P24527
His
C-term
同用名
Leukemia inhibitory factor; Leukotriene A-4 hydrolase; LTA-4 hydrolase; LTA4H
分子量

Approximately 62 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Leukotriene A4 Hydrolase/LTA4H Protein, Mouse (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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