Lgr5/GPR49 蛋白, Human (CHO, hFc)

Lgr5/GPR49 Protein, the receptor for R-spondins, potentiates the canonical Wnt signaling pathway and marks stem cells in the intestinal epithelium and hair follicle. Binding R-spondins (RSPO1-4), Lgr5/GPR49 associates with phosphorylated LRP6 and frizzled receptors, initiating Wnt signaling and amplifying target gene expression. Unlike classical G-protein coupled receptors, Lgr5/GPR49 does not activate heterotrimeric G-proteins, showcasing its unique regulatory role in Wnt signaling. It's crucial for adult intestinal stem cell development and maintenance, identified in a complex with RNF43 and RSPO1, and interacts with RSPO2-4, demonstrating diverse participation in cellular signaling pathways. Lgr5/GPR49 Protein, Human (CHO, hFc) is the recombinant human-derived Lgr5/GPR49 protein, expressed by CHO, with C-hFc labeled tag. The total length of Lgr5/GPR49 Protein, Human (CHO, hFc) is 539 a.a., with molecular weight of 88-110 kDa.

Lgr5/GPR49 蛋白作为 R-spondins 的受体，可增强经典 Wnt 信号通路，同时作为肠上皮和毛囊中独特的干细胞标记。与 R-spondins（RSPO1、RSPO2、RSPO3 或 RSPO4）结合后，Lgr5/GPR49 与磷酸化的 LRP6 和由细胞外 Wnt 激活的卷曲受体结合，启动经典的 Wnt 信号通路并放大靶基因的表达。与经典的 G 蛋白偶联受体相比，Lgr5/GPR49 不会激活异源三聚体 G 蛋白来转导信号，突出了其在 Wnt 信号传导中的独特调节作用。它在胚胎后发育过程中成体肠道干细胞的发育和维持中发挥着至关重要的作用，并已在与 RNF43 和 RSPO1 的复合物中被鉴定。此外，Lgr5/GPR49 与其他 R-spondin 配体（包括 RSPO2、RSPO3 和 RSPO4）相互作用，强调其在细胞信号传导途径中的多样化参与。

Measured by its binding ability in a functional ELISA. Immobilized Human R-Spondin 1 at 5 μg/mL (100 μL/well) can bind Biotinylated Human LGR5. The ED₅₀ for this effect is 42.14 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Human R-Spondin 1 at 5 μg/mL (100 μL/well) can bind Biotinylated Human LGR5.The ED₅₀ for this effect is 42.14 ng/mL.

Human

CHO

C-hFc

O75473 (G22-I560)

8549  [NCBI]

GSSPRSGVLLRGCPTHCHCEPDGRMLLRVDCSDLGLSELPSNLSVFTSYLDLSMNNISQLLPNPLPSLRFLEELRLAGNALTYIPKGAFTGLYSLKVLMLQNNQLRHVPTEALQNLRSLQSLRLDANHISYVPPSCFSGLHSLRHLWLDDNALTEIPVQAFRSLSALQAMTLALNKIHHIPDYAFGNLSSLVVLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPTAIRTLSNLKELGFHSNNIRSIPEKAFVGNPSLITIHFYDNPIQFVGRSAFQHLPELRTLTLNGASQITEFPDLTGTANLESLTLTGAQISSLPQTVCNQLPNLQVLDLSYNLLEDLPSFSVCQKLQKIDLRHNEIYEIKVDTFQQLLSLRSLNLAWNKIAIIHPNAFSTLPSLIKLDLSSNLLSSFPITGLHGLTHLKLTGNHALQSLISSENFPELKVIEMPYAYQCCAFGVCENAYKISNQWNKGDNSSMDDLHKKDAGMFQAQDERDLEDFLLDFEEDLKALHSVQCSPSPGPFKPCEHLLDGWLI

88-110 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.