1. 重组蛋白
  2. Cytokines and Growth Factors
  3. TNF Superfamily
  4. TNF Superfamily Ligands
  5. LIGHT Proteins
  6. LIGHT/TNFSF14 Trimer 蛋白, Human (HEK293, His-Flag)

LIGHT/TNFSF14 Trimer 蛋白, Human (HEK293, His-Flag)

目录号: HY-P78477
产品使用指南

LIGHT蛋白 (CD258; TNFRSF14) 是由活化 T 细胞产生的 II 型跨膜蛋白,是 TNFRSF14/HVEM 配体,属于肿瘤坏死因子 (TNF) 家族。LIGHT 蛋白主要在脾脏中表达,具有膜结合型和可溶性型两种亚型。LIGHT蛋白可以作为肿瘤的免疫检查点分子,并刺激自然杀伤细胞产生干扰素 TFNγ,触发肿瘤凋亡信号。LIGHT 蛋白还参与 LTβR-NIK-p52 NF-κB 的显性通路促进炎症基因表达。此外,LIGHT 蛋白是激活淋巴样细胞的共刺激因子,对疱疹病毒感染有抑制作用。人 LIGHT/TNFSF14 蛋白含有 240 个氨基酸和一个跨膜结构域 (38-58 a.a.)。重组人 LIGHT/TNFSF14 蛋白三聚体 (HEK293, His-Flag) 是人 LIGHT/TNFSF14 蛋白的胞外部分 (S89-V240),由 HEK293 细胞表达,具有 N 端 His 和 Flag 标记。

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100 μg   询价  

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描述

LIGHT/TNFSF14 protein (CD258; TNFRSF14) is a type II transmembrane protein produced by activated T cells. It is a TNFRSF14/HVEM ligand and belongs to the tumor necrosis factor (TNF) family. LIGHT/TNFSF14 is mainly expressed in spleen, with two subtypes: membrane-bound type and soluble type [1]. LIGHT/TNFSF14 protein can be used as immune checkpoint molecule of tumor, and stimulate natural killer cells to produce interferon TFNγ, triggering tumor apoptosis signal [2]. LIGHT/TNFSF14 is also involved in the dominant LTβR-NIK-p52 NF-κB pathway promoting the expression of inflammatory gene[3]. In addition, LIGHT/TNFSF14 protein is a co-stimulatory factor that activates lymphoid cells and has an inhibitory effect on herpes virus infection[4]. Human LIGHT/TNFSF14 Protein has 240 amino acids and a transmembrane domain (38-58 a.a.). LIGHT/TNFSF14 Trimer Protein, Human (HEK293, His-Flag) is the extracellular part (S89-V240) of human LIGHT/TNFSF14 Protein, produced by HEK293 cells, with N-terminal His- and Flag-tag.

研究背景

LIGHT/TNFSF14 is a type II transmembrane protein produced by activated T cells, belongs to tumor necrosis factor (TNF) family. LIGHT/TNFSF14 is a TNFRSF14/HVEM (herpesvirus entry mediator) ligand, engages the receptor for the LTalphabeta heterotrimer but does not form complexes with either secreted lymphotoxin alpha (LTalpha) or LTbeta[1].
LIGHT/TNFSF14 is predominantly expressed in the spleen but also found in the brain. It is weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines[1].
LIGHT/TNFSF14 has a transmemberane, thus it can be leaved into 2 chains: membrane form and soluble form. The soluble form of isoform 1 derives from the membrane form by proteolytic processing.
In tumor immunology, TNFSF14/LIGHT also serves as a novel immune checkpoint molecule for glioblastoma multiforme (GBM), as well as lung carcinoma, breast carcinoma, cervical cancer, and prostate cancer. TNFSF14/LIGHT can stimulate NK cells to produce IFNγ via nuclear factor-κB (NFκB) RelA/p50 signaling. TNFSF14/LIGHT sustains the function of CD8+ effector T cells, trigger apoptosis of various tumor cells[2].
In cell signaling, TNFSF14/LIGHT binds to lymphotoxin-β receptor (LTβR) and HVEM for activating both of them, and disrupts the HVEM-BTLA complex in surface-bound form, and facilitates HVEM-BTLA complex formation in the soluble form[2].
TNFSF14/LIGHT promotes an inflammatory esophageal fibroblast in vitro via a LTβR-NIK-p52 NF-κB dominant pathway with promoting inflammatory gene expression and down-regulating homeostatic factors including WNTs, BMPs and type 3 semaphorins[3].
Beside that, TNFSF14/LIGHT protein is a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. TNFSF14/LIGHT also prevents tumor necrosis factor alpha mediated apoptosis in primary hepatocyte[4][5].

体内研究
(In Vivo)

Exogenous LIGHT/TNFSF14 (human; 100 ng/mL; 24 h, 48 h, and 96 h) effectively reverses the inhibitory effects of miR-326 overexpression on the expression levels of collagen I and fibronectin, and promotes cell proliferation of TGF-β1-treated airway smooth muscle cells (ASMCs)[6].
LIGHT/TNFSF14 (human; 100 ng/mL; 24 h) induces increased expression of CD40, CD86, and HLA-DR in stimulation of iDCs (generated in vitro from CD14+ monocytes)[7].

生物活性

Immobilized Human HVEM hFc at 0.5 μg/mL (100 μL/Well).Dose response curve for Human LIGHT Trimer His with the EC50 of 0.24 μg/mL determined by ELISA.

种属

Human

表达系统

HEK293

标签

N-His;N-Flag

蛋白编号

O43557 (S89-V240)

中文名
重组人 LIGHT/TNFSF14 蛋白三聚体 (HEK293, His-Flag)
同用名
CD258; TNFSF14; HVEM-L; LIGHT; LTg;  TR2
分子量

53-140 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

Solution.

组分

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
参考文献

LIGHT/TNFSF14 Trimer Protein, Human (HEK293, His-Flag) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
LIGHT/TNFSF14 Trimer Protein, Human (HEK293, His-Flag)
目录号:
HY-P78477
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