Livin/BIRC7 蛋白, Human (His)

Livin/BIRC7, an apoptotic regulator, orchestrates proapoptotic and anti-apoptotic activities, crucial for apoptosis, cell proliferation, and cell cycle control. Its anti-apoptotic function involves inhibiting CASP3, CASP7, and CASP9, alongside E3 ubiquitin-protein ligase activity. Despite being a weak caspase inhibitor, Livin's anti-apoptotic prowess arises from ubiquitinating DIABLO/SMAC, preventing its interference with XIAP/BIRC4-caspase interactions, ensuring cell survival. Livin shields against TNF or chemical-induced apoptosis through MAPK8/JNK1 activation, possibly aided by MAP3K7/TAK1 and TAB1. It inhibits CASP3, blocks pro-CASP9 activation, and promotes NK cell-mediated killing, highlighting its multifaceted regulatory roles. Livin/BIRC7 Protein, Human (His) is the recombinant human-derived Livin/BIRC7 protein, expressed by E. coli, with C-His labeled tag. The total length of Livin/BIRC7 Protein, Human (His) is 298 a.a., with molecular weight of ~35 kDa.

Livin/BIRC7 是一种凋亡调节因子，可复杂地调节促凋亡和抗凋亡活性，在细胞凋亡、细胞增殖和细胞周期控制中发挥关键作用。其抗凋亡能力源于对 CASP3、CASP7 和 CASP9 的抑制，以及 E3 泛素蛋白连接酶活性。尽管 Livin 被归类为弱 caspase 抑制剂，但其抗凋亡功能归因于其泛素化 DIABLO/SMAC 的能力，引导其降解，从而促进细胞存活。此外，Livin 可能会阻碍 DIABLO/SMAC 破坏 XIAP/BIRC4-caspase 相互作用，从而可能有助于 caspase 抑制。值得注意的是，Livin 可防止 TNF 或阿霉素、依托泊苷或十字孢菌素等化学制剂诱导的细胞凋亡。这种保护作用是通过激活 MAPK8/JNK1 以及可能的 MAPK9/JNK2 来促进的，具体取决于 TAB1 和 MAP3K7/TAK1。Livin 的多方面功能还包括在体外抑制 CASP3 和阻碍 pro-CASP9 的蛋白水解激活，以及阻止星形孢菌素诱导的细胞凋亡。此外，Livin 促进自然杀伤 (NK) 细胞介导的杀伤，强调其在细胞过程中的多种调节作用。

Measured by its ability to inhibit DEVD-AFC cleavage activity in cell extracts activated by addition of cytochrome c and dATP. The IC₅₀ value is 1.223 nM, as measured under the described conditions.

Human

E. coli

C-His

Q96CA5 (M1-S298)

79444  [NCBI]

MGPKDSAKCLHRGPQPSHWAAGDGPTQERCGPRSLGSPVLGLDTCRAWDHVDGQILGQLRPLTEEEEEEGAGATLSRGPAFPGMGSEELRLASFYDWPLTAEVPPELLAAAGFFHTGHQDKVRCFFCYGGLQSWKRGDDPWTEHAKWFPSCQFLLRSKGRDFVHSVQETHSQLLGSWDPWEEPEDAAPVAPSVPASGYPELPTPRREVQSESAQEPGGVSPAEAQRAWWVLEPPGARDVEAQLRRLQEERTCKVCLDRAVSIVFVPCGHLVCAECAPGLQLCPICRAPVRSRVRTFLS

Approximately 35 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 200 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.