Lymphocyte antigen 6E/LY6E 蛋白, Human (His-SUMO)

Lymphocyte antigen 6E/LY6E protein is a GPI-anchored cell surface regulator that regulates T cell receptor signaling through interaction with CD3Z/CD247. It limits the entry of human coronaviruses, serves as the primary receptor for syncytin-A, and may modulate nicotinic acetylcholine receptor activity. Lymphocyte antigen 6E/LY6E Protein, Human (His-SUMO) is the recombinant human-derived Lymphocyte antigen 6E/LY6E protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of Lymphocyte antigen 6E/LY6E Protein, Human (His-SUMO) is 81 a.a., with molecular weight of ~24.5 kDa.

LY6E 是一种糖基磷脂酰肌醇 (GPI) 锚定的细胞表面蛋白，是 T 淋巴细胞增殖、分化和激活的关键调节因子。从功能上讲，它通过与质膜上的 CD3Z/CD247 成分相互作用来调节 T 细胞受体 (TCR) 信号传导，从而影响 CD3Z/CD247 的磷酸化状态。此外，LY6E 通过破坏刺突蛋白介导的膜融合，在限制人类冠状病毒（包括 SARS-CoV、MERS-CoV 和 SARS-CoV-2）的进入方面发挥着关键作用。值得注意的是，它作为合胞素-A (SynA) 的主要受体，通过促进合体滋养层 I (SynT-I) 的融合并确保胎盘内胎儿和母体脉管系统的正确形态发生来促进胎盘形成。此外，LY6E 可能充当烟碱乙酰胆碱受体 (nAChR) 活性的调节剂。在微生物感染的情况下，LY6E既促进又限制病毒进入，增强了各种病毒的融合过程，包括HIV-1、西尼罗河病毒、登革热病毒和寨卡病毒，同时对于从入口进入的副粘病毒PIV5来说是可有可无的。质膜。从机制上讲，LY6E 在病毒感染时采用微管样组织，有助于增强内体逃逸后的病毒脱壳。

Human

E. coli

N-His;N-SUMO

Q16553 (21L-101S)

4061  [NCBI]

LMCFSCLNQKSNLYCLKPTICSDQDNYCVTVSASAGIGNLVTFGHSLSKTCSPACPIPEGVNVGVASMGISCCQSFLCNFS

Approximately 24.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in Tris-based buffer, 50% glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.