1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Chemokine & Receptors
  4. CC Chemokines
  5. MCP-1/CCL2
  6. MCP-1/CCL2 蛋白, Mouse (HEK293)

MCP-1/CCL2 蛋白, Mouse (HEK293)

目录号: HY-P7235
COA 产品使用指南

MCP-1/CCL2 蛋白, Mouse (HEK293) 是属于 CC 趋化因子家族的细胞因子,可以与细胞表面的CCR2趋化因子受体相互作用,介导炎症免疫反应、病毒感染以及肿瘤发生等。MCP-1/CCL2 蛋白, Mouse (HEK293) 是一种由 HEK293 表达的重组小鼠 MCP-1/CCL2 (Q24-R96)。

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2 μg ¥700 In-stock
10 μg ¥1950 In-stock
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100 μg ¥7500 In-stock
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描述

MCP-1/CCL2 Protein, Mouse (HEK293) is a cytokine belonging to the CC chemokine family that interacts with the CCR2 chemokine receptor on the cell surface to mediate inflammatory immune responses, viral infections, and tumorigenesis. MCP-1/CCL2 Protein, Mouse (HEK293) is a mouse MCP-1/CCL2 (Q24-R96) expressed by HEK293[1].

研究背景

CCL2,也称为单核细胞趋化蛋白1 (MCP1),是属于CC趋化因子家族的小细胞因子。CCL2基因位于人类17号染色体的q11.2-q12区域,编码分子量为9-15 kDa的单体多肽,具体取决于糖基化水平。CCL2主要由单核细胞、巨噬细胞和树突状细胞分泌。由单核细胞、巨噬细胞和树突状细胞分泌,血小板源性生长因子是CCL2基因的主要诱导剂。星形胶质细胞和小胶质细胞也被认为是 CCL2[1] 的来源。CCL2 通过结合和激活 CCR2 发出信号,并诱导强烈的趋化反应和钙离子的细胞内动员。其中,CCL2/CCR2可以通过激活β1整合素和p38-MAPK信号通路来调节巨噬细胞的细胞粘附和趋化性。除了充当化学引诱剂之外,CCL2 还可以通过改变紧密连接 (TJ) 蛋白、调节内皮粘附分子和白细胞整合素的表达以及细胞因子的产生来调节体外脑内皮通透性。此外,CCL2-CCR2 信号传导轴与许多炎症和神经退行性疾病有关,其作用是将炎症细胞招募到 CNS[2]。CCL2 最初被描述为“肿瘤源性趋化因子”,现已被证明是多种类型免疫细胞的有效趋化因子,也是治疗多种疾病的潜在靶标,例如动脉粥样硬化、多发性硬化症、哮喘、神经性疼痛、糖尿病肾病和癌症[3]

体外研究
(In Vitro)

MCP-1/CCL2 (100 ng/mL, 24 h) can induce NO production by co-treatment with IFN-γ, increases ERK phosphorylation and increases iNOS expression, suggesting that the mechanism of NO production is related to the ERK1/2 signaling pathway in peritoneal macrophages of mice, thereby increasing bacterial clearance[3].
 CCL2 (0.05 ng/μL) promotes α-synuclein secretion and α-synuclein-induced neuronal apoptosis, and induces microglia proliferation and secretion of TNF-α, IL-1β and NO[4].

体内研究
(In Vivo)

MCP-1/CCL2 (intracerebral injection, 5-25 μg, 1 μL/h for 3 days or 0.5 μL/h for 7 days) induces FITC-albumin leakage at lower concentrations (5-20 μg) but fails or moderately induces leukocyte infiltration. Blood-brain barrier permeability and leukocyte infiltration are significantly increased at 25 μg, and extravasation is significantly enhanced in the treated CD-1 mice compared with the control group 6 h after injection. Prolonged administration for 3 or 7 days results in a significant increase in the percentage of brain water content, and decreases expression of the tight junction proteins occludin, claudin-5, ZO-1 and ZO-2[2].

生物活性

1. The ED50 is <0.3 μg/mL as measured by CHO-K1/Gα15/mCCR2 cells (human Gα15 and mouse CCR2 stably expressed in CHO-K1 cells).
2. Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED50 this effect is <26.8 ng/mL, corresponding to a specific activity is >3.733×10^4 U/mg.

  • Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED50 for this effect is 26.79 ng/mL, corresponding to a specific activity is 3.733×104 U/mg.
种属

Mouse

表达系统

HEK293

标签

Tag Free

蛋白编号

P10148 (Q24-R96)

基因 ID

20296  [NCBI]

蛋白结构
N-term
CCL2 (Q24-R96)
Accession # P10148
C-term
中文名
重组小鼠MCP-1/CCL2蛋白(HEK293)
同用名
rMuMCP-1/CCL2; C-C motif chemokine 2; MCAF; MCP-1; SCYA2
氨基酸序列

QPDAVNAPLTCCYSFTSKMIPMSRLESYKRITSSRCPKEAVVFVTKLKREVCADPKKEWVQTYIKNLDRNQMR

分子量

Approximately 11.85 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

MCP-1/CCL2 Protein, Mouse (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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