MCP-3/CCL7 蛋白, Human

MCP-3/CCL7 Protein, Human  is a CC chemokine and elicitor that binds to CCR1, CCR2 and CCR3 to mediate antiviral, antibacterial, antitumor and other immune responses. MCP-3/CCL7 Protein, Human  is a recombinant human MCP-3/CCL7 protein expressed by E.coilMCP-3/CCL7 (Q24-L99)^[1].

CCL7，也称为单核细胞趋化蛋白3 (MCP3)，是一种小细胞因子。在人类基因组中，CCL7由位于染色体17q11.2-q12上的CCL7基因编码。它由99个氨基酸组成，其中包括23个氨基酸的信号肽，而信号肽裂解后分泌约76个氨基酸的成熟蛋白。CCL7 在多种细胞类型中表达，如基质细胞、角蛋白形成细胞、气道平滑肌细胞、实质细胞、成纤维细胞和白细胞以及肿瘤细胞^[1]。CCL7通常以单体形式存在，可以与多种受体结合，包括CCR1、CCR2、CCR3、CCR5和CCR10，从而介导对免疫细胞类型的影响。CCL7可以充当趋化剂，吸引多种白细胞，包括单核细胞和白细胞。中性粒细胞。它通过将白细胞募集到感染组织来介导免疫反应，还参与单核细胞动员和募集单核细胞到炎症部位，以及通过增加细胞内 Ca²⁺ 诱导中性粒细胞迁移到炎症部位。通量。CCL7 参与抗菌、抗病毒和抗真菌免疫反应，并与多种免疫疾病有关，如溃疡性结肠炎、多发性硬化症或非特应性和特应性哮喘。同时，CCL7 表达可激活抗肿瘤免疫反应^[2]。

CCL7(10 and 100 ng/mL, 6 h) can dose-dependently and significantly increase oral squamous cell carcinoma (OSCC) cell migration into the wound and induce cytoskeletal modifications in OSCC cells^[3].

1. Full biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/mL.
2. Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ this effect is 0.08829 μg/mL, corresponding to a specific activity is 1.132×10⁴ U/mg.

• 
  Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 0.08829 μg/mL, corresponding to a specific activity is 1.132×10⁴ U/mg.

Human

E. coli

Tag Free

P80098 (Q24-L99)

6354  [NCBI]

QPVGINTSTTCCYRFINKKIPKQRLESYRRTTSSHCPREAVIFKTKLDKEICADPTQKWVQDFMKHLDKKTQTPKL

Approximately 12.97 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. G Opdenakker, et al. The human MCP-3 gene (SCYA7): cloning, sequence analysis, and assignment to the C-C chemokine gene cluster on chromosome 17q11.2-q12. Genomics. 1994 May 15;21(2):403-8.  [Content Brief]

  [2]. F Fioretti, et al. Reduced tumorigenicity and augmented leukocyte infiltration after monocyte chemotactic protein-3 (MCP-3) gene transfer: perivascular accumulation of dendritic cells in peritumoral tissue and neutrophil recruitment within the tumor. J Immunol. 1998 Jul 1;161(1):342-6.  [Content Brief]

  [3]. Da-Woon Jung, et al.  Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7. Int J Cancer. 2010 Jul 15;127(2):332-44.  [Content Brief]