Menin 蛋白, Human

Menin Protein is an endocrine tumor suppressor and transcriptional regulator. By binding to various transcription factors and signaling molecules, Menin Protein plays roles in activating or inhibiting gene transcription and regulating multiple signaling pathways such as TGFβ and Wnt in different tissues. Menin Protein is crucial in processes including cell growth, cell cycle, genomic stability, and bone development. Menin Protein, Human is a recombinant Menin protein expressed by E. coli without a tag^[1][2][3].

Menin 蛋白由 MEN1 基因编码，可通过结合转录激活因子 (如 MLL1、c-Myb) 和抑制因子 (如 JunD、NFκB) 调控基因转录，既可通过招募组蛋白修饰酶促进 H3K4me3 等激活基因表达，也能通过募集 HDAC、EZH2 等抑制转录。Menin 蛋白参与 TGFβ/SMAD、Wnt/β-catenin、Ras/ERK 等多条信号通路，在不同组织中功能迥异，如在胰岛细胞中抑制增殖、在白血病细胞中协同 MLL 融合蛋白驱动 HOX 基因表达促癌。此外，Menin 蛋白自身受磷酸化、SUMO 化等翻译后修饰及催乳素、TGFβ 等信号通路调控，其结构中的中央口袋是结合多种蛋白的关键功能域^[1][2][3]。

过表达 Menin 在妊娠 βMen1 小鼠中导致胰岛 β 细胞增殖减少、血糖升高和葡萄糖耐量受损^[3]。

Human

E. coli

Tag Free

O00255-1 (M1-L615)

/

Full Length

Approximately 68 kDa

Greater than 85% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Balogh K, et al. Menin and its interacting proteins: elucidation of menin function. Trends Endocrinol Metab. 2006 Nov;17(9):357-64.  [Content Brief]

  [2]. Matkar S, et al. Menin: a scaffold protein that controls gene expression and cell signaling. Trends Biochem Sci. 2013 Aug;38(8):394-402.  [Content Brief]

  [3]. Karnik SK, et al. Menin controls growth of pancreatic beta-cells in pregnant mice and promotes gestational diabetes mellitus. Science. 2007 Nov 2;318(5851):806-9.  [Content Brief]