1. 重组蛋白
  2. Receptor Proteins
  3. Cell Adhesion Molecules (CAMs)
  4. Mesothelin 蛋白, Human (250a.a, HEK293, His)

Mesothelin 蛋白, Human (250a.a, HEK293, His)

目录号: HY-P70413
COA 产品使用指南

间皮素蛋白,尤其是其膜锚定形式,可能介导细胞粘附,暗示其在细胞相互作用中的作用。此外,相关的巨核细胞增强因子(MPF)积极增强体外巨核细胞集落的形成,表明其参与促进特化血细胞集落的发育。Mesothelin 蛋白, Human (250a.a, HEK293, His) 是重组的 间皮素蛋白,由 HEK293 表达,带有 C-6*His 标签。Mesothelin 蛋白, Human (250a.a, HEK293, His) 全长 250 个氨基酸,分子量为 30-35 kDa。

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规格 价格 是否有货 数量
10 μg ¥720 In-stock
50 μg ¥2000 In-stock
100 μg   询价  

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描述

Mesothelin Protein, especially in its membrane-anchored forms, potentially mediates cellular adhesion, implicating its role in cell interactions. Furthermore, the associated Megakaryocyte-potentiating factor (MPF) actively enhances in vitro megakaryocyte colony formation, indicating its participation in fostering specialized blood cell colonies' development. Mesothelin Protein, Human (250a.a, HEK293, His) is the recombinant human-derived Mesothelin protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Mesothelin Protein, Human (250a.a, HEK293, His) is 250 a.a., with molecular weight of 30-35 kDa.

研究背景

间皮素蛋白,特别是其膜锚定形式,在细胞粘附中表现出潜在作用,表明参与介导细胞之间的相互作用。此外,相关的巨核细胞增强因子(MPF)已被确定为体外巨核细胞集落形成的有效增强剂,表明其积极参与促进这些特殊血细胞集落的发育。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q13421 (L37-R286)

基因 ID
蛋白结构
N-term
Mesothelin (L37-R286)
Accession # Q13421
6*His
C-term
同用名
rHuMesothelin/MPF, His ; Megakaryocyte potentiating factor; mesothelin; Pre-pro-megakaryocyte-potentiating factor; soluble MPF mesothelin related protein; CAK1; MPF; MSLN; SMR; CAK1; CAK1 antigen
氨基酸序列

LAGETGQEAAPLDGVLANPPNISSLSPRQLLGFPCAEVSGLSTERVRELAVALAQKNVKLSTEQLRCLAHRLSEPPEDLDALPLDLLLFLNPDAFSGPQACTRFFSRITKANVDLLPRGAPERQRLLPAALACWGVRGSLLSEADVRALGGLACDLPGRFVAESAEVLLPRLVSCPGPLDQDQQEAARAALQGGGPPYGPPSTWSVSTMDALRGLLPVLGQPIIRSIPQGIVAAWRQRSSRDPSWRQPER

分子量

30-35 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料

Mesothelin Protein, Human (250a.a, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Mesothelin Protein, Human (250a.a, HEK293, His)
目录号:
HY-P70413
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