1. 重组蛋白
  2. Enzymes & Regulators
  3. Matrix Metalloproteinases
  4. MMP-2
  5. MMP-2 蛋白, Mouse (HEK293, His)

MMP-2 蛋白, Mouse (HEK293, His)

目录号: HY-P73810
COA 产品使用指南

MMP-2 蛋白是一种普遍存在的金属蛋白酶,有助于脉管系统重塑、血管生成、组织修复、肿瘤侵袭、炎症和动脉粥样硬化斑块破裂。除了细胞外基质降解外,它还针对非基质蛋白并促进血管收缩。MMP-2 蛋白, Mouse (HEK293, His) 是重组的 MMP-2 蛋白,由 HEK293 表达,带有 N-His 标签。MMP-2 蛋白, Mouse (HEK293, His) 全长 254 个氨基酸,分子量约为 ~34.54 kDa。

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规格 价格 是否有货 数量
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5 μg ¥980 In-stock
10 μg ¥1560 In-stock
20 μg ¥2500 In-stock
50 μg ¥4750 In-stock
100 μg ¥7600 In-stock
500 μg ¥21000 In-stock
> 100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

MMP-2 protein is a ubiquitous metalloprotease that contributes to vasculature remodeling, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. In addition to extracellular matrix degradation, it also targets non-matrix proteins and promotes vasoconstriction. MMP-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived MMP-2 protein, expressed by HEK293 , with N-His labeled tag. The total length of MMP-2 Protein, Mouse (HEK293, His) is 254 a.a., with molecular weight of ~34.54 kDa.

研究背景

MMP-2 蛋白是一种多功能、普遍存在的金属蛋白酶,参与多种功能,包括脉管系统重塑、血管生成、组织修复、肿瘤侵袭、炎症和动脉粥样硬化斑块破裂。除了降解细胞外基质蛋白外,它还作用于大内皮细胞 1 和 β 型 CGRP 等非基质蛋白,促进血管收缩。此外,它在 Gly-|-Leu 键处裂解 KISS,似乎通过影响 GSK3beta 活性在心肌细胞死亡途径和心肌氧化应激调节中发挥作用。此外,MMP-2 参与纤维血管组织的形成,其 C 端非催化片段 PEX 具有抗血管生成和抗肿瘤特性,同时抑制细胞迁移和对 FGF2 和玻连蛋白的粘附。此外,MMP-2 作为血管表面整合素 α-v/β-3 的配体。

生物活性

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is 4214 pmol/min/µg, as measured under the described conditions.

种属

Mouse

表达系统

HEK293

标签

N-His

蛋白编号

P33434 (M409-C662)

基因 ID
蛋白结构
N-term
His
MMP-2 (M409-C662)
Accession # P33434
C-term
同用名
72 kDa Type IV Collagenase; Gelatinase A; MMP-2; TBE-1; CLG4A
氨基酸序列

MGLEHSQDPGALMAPIYTYTKNFRLSHDDIKGIQELYGPSPDADTDTGTGPTPTLGPVTPEICKQDIVFDGIAQIRGEIFFFKDRFIWRTVTPRDKPTGPLLVATFWPELPEKIDAVYEAPQEEKAVFFAGNEYWVYSASTLERGYPKPLTSLGLPPDVQQVDAAFNWSKNKKTYIFAGDKFWRYNEVKKKMDPGFPKLIADSWNAIPDNLDAVVDLQGGGHSYFFKGAYYLKLENQSLKSVKFGSIKSDWLGC

分子量

Approximately 34.54 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 (Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.) or 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

MMP-2 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
MMP-2 Protein, Mouse (HEK293, His)
目录号:
HY-P73810
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