MMP-9 蛋白, Rat (HEK293, His)

Matrix metalloproteinase-9 (Mmp9) is a member of the matrix metalloproteinase (MMP) family. Mmp9 is secreted as inactive proproteins which are activated when cleaved by extracellular proteinases to generate a mature, zinc-dependent endopeptidase enzyme that degrades collagens of type IV, V and XI, and elastin. Mmp9 is also involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow. MMP-9 Protein, Rat (HEK293, His) is the recombinant rat-derived MMP-9 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of MMP-9 Protein, Rat (HEK293, His) is 689 a.a., with molecular weight of approximately 95 kDa.

基质金属蛋白酶-9 (Mmp9) 是基质金属蛋白酶 (MMP) 家族的一员，在胚胎发育、生殖、伤口修复、组织重塑等正常生理过程中参与细胞外基质的分解，也参与疾病过程，如关节炎、动脉粥样硬化进展和肿瘤侵袭等。Mmp9 作为无活性的原蛋白被分泌，当被细胞外蛋白酶裂解产生成熟的锌依赖性内肽酶时被激活，可降解 IV 型、V 型和 XI 型胶原蛋白和弹性蛋白。Mmp9 还参与 IL-8 诱导的骨髓造血祖细胞的动员。Mmp9 与多种疾病有关，包括唐氏综合症、无牙、动脉疾病、自身免疫性疾病和慢性阻塞性肺病^[1][2]。

Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 and the specific activity is >1000 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by APMA for an activated form)

Rat

HEK293

C-10*His

EDL96479.1 (A20-P708)

81687  [NCBI]

APHQRQPTYVVFPRDLKTSNLTDTQLAEDYLYRYGYTRAAQMMGEKQSLRPALLMLQKQLSLPQTGELDSETLKAIRSPRCGVPDVGKFQTFEGDLKWHHHNITYWIQSYTEDLPRDVIDDSFARAFAVWSAVTPLTFTRVYGLEADIVIQFGVAEHGDGYPFDGKDGLLAHAFPPGPGIQGDAHFDDDELWSLGKGAVVPTYFGNANGAPCHFPFTFEGRSYLSCTTDGRNDGKPWCGTTADYDTDRKYGFCPSENLYTEHGNGDGKPCVFPFIFEGHSYSACTTKGRSDGYRWCATTANYDQDKLYGFCPTRADVTVTGGNSAGEMCVFPFVFLGKQYSTCTGEGRSDGRLWCATTSNFDADKKWGFCPDQGYSLFLVAAHEFGHALGLDHSSVPEALMYPMYHYHEDSPLHEDDIKGIQHLYGRGSKPDPRPPATTAAEPQPTAPPTMCPTAPPMAYPTGGPTVAPTGAPSPGPTGPPTAGPSEAPTESSTPVDNPCNVDVFDAIADIQGALHFFKDGRYWKFSNHGGSQLQGPFLIARTWPALPAKLNSAFEDPQSKKIFFFSGRKMWVYTGQSVLGPRSLDKLGLGSEVTLVTGLLPRRGGKALLISRERIWKFDLKSQKVDPQSVTRLDNEFSGVPWNSHNVFHYQDKAYFCHDKYFWRVSFHNRVNQVDHVAYVTYDLLQCP

approximately 95 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM MES, 100 mM NaCl, 1 mM CaCl₂, 10% Glycerol, pH 7.4 (Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.) or 50 mM MES, 100 mM NaCl, 1 mM CaCL₂, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.