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  3. NAMPT 蛋白, Mouse (P.pastoris, His)

NAMPT 蛋白, Mouse (P.pastoris, His)

目录号: HY-P701315
产品使用指南

NAMPT蛋白具有细胞因子和脂肪因子的双重功能。由于 ATP 活化有限,其分泌形式缺乏酶活性。它催化烟酰胺和 5-磷酸核糖基-1-焦磷酸缩合生成烟酰胺单核苷酸,这对于 NAD 生物合成至关重要。NAMPT 蛋白, Mouse (P.pastoris, His) 是重组的 NAMPT 蛋白,由 P. pastoris 表达,带有 N-10*His 标签。NAMPT 蛋白, Mouse (P.pastoris, His) 全长 491 个氨基酸,分子量为 57.9 kDa。

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描述

NAMPT protein has dual functions of cytokine and adipokine. Due to limited ATP activation, its secreted form lacks enzymatic activity. It catalyzes the condensation of nicotinamide and 5-phosphoribosyl-1-pyrophosphate to produce nicotinamide mononucleotide, which is critical for NAD biosynthesis. NAMPT Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived NAMPT protein, expressed by P. pastoris , with N-10*His labeled tag. The total length of NAMPT Protein, Mouse (P.pastoris, His) is 491 a.a., with molecular weight of 57.9 kDa.

研究背景

NAMPT 蛋白具有双重功能,既可作为具有免疫调节特性的细胞因子,又可作为具有抗糖尿病特性的脂肪因子。有趣的是,其分泌形式缺乏酶活性,部分原因是由于其水平较低,细胞外空间和血浆中 ATP 的激活有限。从功能上来说,NAMPT 催化烟酰胺与 5-磷酸核糖基-1-焦磷酸的缩合,产生烟酰胺单核苷酸——NAD 生物合成中至关重要的中间体。NAMPT 作为哺乳动物 NAD 生物合成途径中的限速成分,在调节生物钟功能中发挥着关键作用。其 NAMPT 依赖性振荡产生 NAD,通过从 NAD 依赖性 SIRT1 介导的抑制中释放核心时钟成分 CLOCK-BMAL1 异二聚体,控制时钟靶基因表达的振荡。

种属

Mouse

表达系统

P. pastoris

标签

N-10*His

蛋白编号

Q99KQ4 (M1-H491)

基因 ID

59027

蛋白结构
N-term
10*His
NAMPT (M1-H491)
Accession # Q99KQ4
C-term
同用名
rHuNicotinamide phosphoribosyltransferase/NAMPT, His; Pre-B cell-enhancing factor; Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt; Pre-B-cell colony-enhancing factor 1; Visfatin; NAMPT; PBEF; PBEF1
分子量

57.9 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

NAMPT Protein, Mouse (P.pastoris, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
NAMPT Protein, Mouse (P.pastoris, His)
目录号:
HY-P701315
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