NAMPT 蛋白, Mouse

NAMPT protein has dual functions of cytokine and adipokine. Due to limited ATP activation, its secreted form lacks enzymatic activity. It catalyzes the condensation of nicotinamide and 5-phosphoribosyl-1-pyrophosphate to produce nicotinamide mononucleotide, which is critical for NAD biosynthesis. NAMPT Protein, Mouse is the recombinant mouse-derived NAMPT protein, expressed by E. coli , with tag free.

NAMPT 蛋白具有双重功能，既可作为具有免疫调节特性的细胞因子，又可作为具有抗糖尿病特性的脂肪因子。有趣的是，其分泌形式缺乏酶活性，部分原因是由于其水平较低，细胞外空间和血浆中 ATP 的激活有限。从功能上来说，NAMPT 催化烟酰胺与 5-磷酸核糖基-1-焦磷酸的缩合，产生烟酰胺单核苷酸——NAD 生物合成中至关重要的中间体。NAMPT 作为哺乳动物 NAD 生物合成途径中的限速成分，在调节生物钟功能中发挥着关键作用。其 NAMPT 依赖性振荡产生 NAD，通过从 NAD 依赖性 SIRT1 介导的抑制中释放核心时钟成分 CLOCK-BMAL1 异二聚体，控制时钟靶基因表达的振荡。

Measured by its ability to induce VEGF secretion by PC-3 human prostate cancer cells. The ED₅₀ for this effect is 1.337 μg/mL.

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  Measured by its ability to induce VEGF secretion by PC-3 human prostate cancer cells. The ED₅₀ for this effect is 1.337 μg/mL, corresponding to a specific activity is 7.48×10² U/mg.

Mouse

E. coli

Tag Free

Q99KQ4 (M1-H491)

59027

Full Length

MNAAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTENSKVRKVKYEETVFYGLQYILNKYLKGKVVTKEKIQEAKEVYREHFQDDVFNERGWNYILEKYDGHLPIEVKAVPEGSVIPRGNVLFTVENTDPECYWLTNWIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASAHLVNFKGTDTVAGIALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTEAPLIIRPDSGNPLDTVLKVLDILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKKWSIENVSFGSGGALLQKLTRDLLNCSFKCSYVVTNGLGVNVFKDPVADPNKRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGHDLLHTVFKNGKVTKSYSFDEVRKNAQLNIEQDVAPH

Approximately 56 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.