1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Noggin 蛋白, Mouse (CHO)

Noggin 蛋白, Mouse (CHO)

目录号: HY-P7086
COA 产品使用指南

Noggin 蛋白, Mouse (CHO)是骨形态发生蛋白 (BMP) 拮抗剂。

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规格 价格 是否有货 数量
10 μg ¥1200 In-stock
50 μg ¥4600 In-stock
100 μg ¥7800 询价
> 100 μg   询价  

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描述

Noggin Protein, Mouse (CHO) is a bone morphogenetic protein (BMP) antagonist.

研究背景

哺乳动物头蛋白的序列与非洲爪蟾头蛋白非常相似,并且在非洲爪蟾胚胎组织上进行的诱导测定中具有相似的活性。在成年哺乳动物中,头蛋白在神经系统中的表达最为显着[1]。Noggin 是一种骨形态发生蛋白 (BMP) 拮抗剂,在 Spemann 组织中表达。鼠Noggin 在浓缩软骨和未成熟软骨细胞中表达。在缺乏 Noggin 的小鼠中,软骨凝结正常启动,但出现增生,关节发育启动失败[2]。Noggin 蛋白以高亲和力结合 BMP4,并可通过阻断与同源细胞表面受体的结合来消除 BMP4 活性[3]。人头蛋白 (hNoggin) 在去除其信号序列(残基 1-27)后有 205 个氨基酸(残基 28-232),并作为糖基化、共价连接的同二聚体分泌。人 Noggin 与 hBMP-4 具有高亲和力(而与 hBMP-7[4] 具有较低亲和力。

生物活性

The ED50 is <60 ng/mL as measured by ATDC5 cells.

种属

Mouse

表达系统

CHO

标签

Tag Free

蛋白编号

P97466 (L20-C232)

基因 ID

18121  [NCBI]

蛋白结构
N-term
Noggin (L20-C232)
Accession # P97466
C-term
同用名
rMuNoggin; NOG
氨基酸序列

LRAAPAGGQHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGPAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRCQRRGGQRCGWIPIQYPIISECKCSC

分子量

29-31 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized after extensive dialysis against PBS.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

Noggin Protein, Mouse (CHO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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