Noggin 蛋白, Mouse (HEK293, His)

Noggin is an indispensable factor in cartilage morphogenesis and joint formation, a potent inhibitor of bone morphogenetic protein (BMP) signaling, and plays a crucial role in the growth and patterning of the neural tube and somites. By forming homodimers, Noggin acts as a key regulator to inhibit chondrocyte differentiation by interacting with growth and differentiation factors, particularly GDF5 and possibly GDF6. Noggin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Noggin protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Noggin Protein, Mouse (HEK293, His) is 205 a.a., with molecular weight of ~30.0 kDa.

Noggin 是一种参与软骨形态发生和关节形成的重要蛋白质，在抑制骨形态发生蛋白 (BMP) 信号传导方面发挥着至关重要的作用，而骨形态发生蛋白 (BMP) 对于神经管和体节的生长和模式形成至关重要。作为 BMP 的抑制剂，Noggin 通过与生长和分化因子 5 (GDF5) 以及可能的 GDF6 相互作用，参与软骨细胞分化的调节。Noggin 作为同二聚体存在，直接与 GDF5 相互作用，导致软骨细胞分化受到抑制。这些多方面的功能强调了 Noggin 在胚胎发育中的重要性，特别是在骨骼和神经组织形成的复杂过程中，并强调了它作为 BMP 信号通路关键调节剂的作用（参考：提供的信息）。

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is ≤0.1785 μg/mL.

• 
  Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.1785 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4,corresponding to a specific activity is 5.602×10³ units/mg.

Mouse

HEK293

C-6*His

P97466 (Q28-C232)

18121  [NCBI]

QHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGPAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRCQRRGGQRCGWIPIQYPIISECKCSC

Approximately 30.0 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, 5 mM EDTA, 5% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.