1. 重组蛋白
  2. Enzymes & Regulators Ubiquitin Related Proteins
  3. Transferases (EC 2) Ubiquitin Enzymes
  4. E3 Ligases
  5. NOSIP 蛋白, Human (His)

NOSIP 蛋白, Human (His)

目录号: HY-P701598
产品使用指南

NOSIP 是一种 E3 泛素蛋白连接酶,在前脑、眼睛和面部的正常发育中发挥着关键作用。值得注意的是,它催化丝氨酸/苏氨酸蛋白磷酸酶 2A (PP2A) 催化亚基的单泛素化,特别是 PPP2CA/PPP2CB。NOSIP 蛋白, Human (His) 是重组的 NOSIP 蛋白,由 E. coli 表达,带有 N-6*His 标签。NOSIP 蛋白, Human (His) 全长 300 个氨基酸。

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描述

NOSIP is an E3 ubiquitin protein ligase that plays a key role in the normal development of the forebrain, eye, and face. Notably, it catalyzes the monoubiquitination of the serine/threonine protein phosphatase 2A (PP2A) catalytic subunit, specifically PPP2CA/PPP2CB. NOSIP Protein, Human (His) is the recombinant human-derived NOSIP protein, expressed by E. coli , with N-6*His labeled tag. The total length of NOSIP Protein, Human (His) is 300 a.a., .

研究背景

NOSIP 是一种重要的 E3 泛素蛋白连接酶,对于前脑、眼睛和面部的正常发育至关重要。它通过催化丝氨酸/苏氨酸蛋白磷酸酶 2A (PP2A) 催化亚基 PPP2CA/PPP2CB 的单泛素化,在细胞过程中发挥关键作用。此外,NOSIP 通过诱导 NOS1 和 NOS3 易位至肌动蛋白细胞骨架,最终抑制其酶活性,从而充当一氧化氮生成的负调节因子。这种双重功能凸显了 NOSIP 在发育过程和一氧化氮信号传导调节中的重要性,强调了其在细胞稳态中的多功能作用。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

Q9Y314 (T2-A301)

基因 ID

51070

蛋白结构
N-term
6*His
NOSIP (T2-A301)
Accession # Q9Y314
C-term
同用名
NOSIP; Nitric oxide synthase-interacting protein; E3 ubiquitin-protein ligase NOSIP; RING-type E3 ubiquitin transferase NOSIP; eNOS-interacting protein
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
NOSIP Protein, Human (His)
目录号:
HY-P701598
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