Osteopontin/OPN 蛋白, Human (HEK293, His)

Osteopontin/OPN is a secretory glycophosphoprotein belonging to the SIBLING family. It contains RGD sequences and phosphorylation sites and can bind to integrins α_vβ₃, α₄β₁ and CD44 receptors. Osteopontin/OPN regulates cell adhesion and migration through receptor-mediated regulation, activates MAPK/ERK and PI3K-AKT to promote tumor invasion, and upregulates TIMP-1/2 to inhibit MMPs and protect cartilage. Osteopontin/OPN can be used in the study of osteoarthritis, liver cancer, etc^[1][2][3].

Osteopontin/OPN 是一种 44-75 kDa 的分泌型糖磷蛋白，属于小整合素结合配体 N-糖蛋白 (SIBLING) 家族，含 RGD (Arg-Gly-Asp) 序列及多个磷酸化位点，可与整合素 α_vβ₃、α₄β₁及 CD44 受体结合^[1][2][3]。
Osteopontin/OPN 通过受体介导调控细胞黏附、迁移、增殖及基质重塑，参与炎症、骨代谢及肿瘤进展。Osteopontin/OPN 可上调 TIMP-1 和 TIMP-2，抑制基质金属蛋白酶 (MMPs) 活性，保护软骨细胞。Osteopontin/OPN 还通过 NF-κB 通路诱导炎症因子释放，参与骨关节炎 (OA) 病理进程^[1]。同时，Osteopontin/OPN 激活 MAPK/ERK 和 PI3K-AKT 通路，促进肿瘤细胞侵袭 (如骨肉瘤 OS-732 细胞迁移)^[2]； Osteopontin/OPN 在上游受 TNF-α、IL-1β 诱导，在下游激活整合素-细胞骨架信号级联^[3]。
Osteopontin/OPN 可用于骨关节炎 (OA) 的研究。关节内 OPN 通过上调 TIMP-1/2 抑制 MMPs，延缓软骨降解^[1]。Osteopontin/OPN 还可用于肿瘤诊断与预后的研究。术前血浆 OPN 水平是肝癌 (HCC) 复发和不良预后的独立预测因子 (临界值 200 ng/mL)^[3]；而反义 OPN RNA 可抑制骨肉瘤细胞致瘤性，延长裸鼠肿瘤潜伏期并减少瘤重^[2]。

Osteopontin/OPN (人源；100 ng/mL、1 μg/mL；48 h) 对人膝骨关节炎软骨细胞无细胞毒性；在 1 μg/mL 浓度下，显著上调人膝骨关节炎软骨细胞中 TIMP-1 和 TIMP-2 mRNA 水平，而 100 ng/mL 组无显著变化^[1]。

Osteopontin/OPN (人源；反义 RNA 转染；细胞移植；27 天) 在 Balb/c 裸鼠骨肉瘤 (OS-732 细胞) 皮下移植模型中，反义 hOPN RNA 转染的 OS-732 细胞显著延长肿瘤潜伏期至 14 天 (对照组为 7 天)，抑制肿瘤生长，降低肿瘤质量^[2]。

Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 10^5 cells/well are added to Recombinant Human Osteopontin coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C. The ED₅₀ for this effect is <0.3 μg/mL.

• 
  Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 10⁵ cells/well are added to Recombinant Human Osteopontin coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C. The ED₅₀ for this effect is 0.1486 μg/mL, corresponding to a specific activity is 6729.475 units/mg.

Human

HEK293

C-6*His

P10451-1 (I17-N314)

6696  [NCBI]

Full Length of Isoform-1 Mature Protein

IPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSNESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELDSASSEVN

Approximately 50-69 kDa due to the glycosylation

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Zhang FJ, et al. Effect of osteopontin on TIMP-1 and TIMP-2 mRNA in chondrocytes of human knee osteoarthritis in vitro. Exp Ther Med. 2014 Aug;8(2):391-394.  [Content Brief]

  [2]. Liu SJ, et al. The inhibition of in vivo tumorigenesis of osteosarcoma (OS)-732 cells by antisense human osteopontin RNA. Cell Mol Biol Lett. 2008;13(1):11-9.  [Content Brief]

  [3]. Zhang H, et al. The prognostic significance of preoperative plasma levels of osteopontin in patients with hepatocellular carcinoma. J Cancer Res Clin Oncol. 2006 Nov;132(11):709-17.  [Content Brief]