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  3. Osteopontin/OPN 蛋白, Human (HEK293, His)

Osteopontin/OPN 蛋白, Human (HEK293, His)

目录号: HY-P70499
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Osteopontin/OPN 是属于 SIBLING 家族的分泌型糖磷蛋白,含 RGD 序列及磷酸化位点,可与整合素 αvβ3、α4β1CD44 受体结合。Osteopontin/OPN 通过受体介导调控细胞黏附、迁移等,激活 MAPK/ERK 和 PI3K-AKT 促进肿瘤侵袭,上调 TIMP-1/2 抑制 MMPs 保护软骨,可用于骨关节炎、肝癌等研究。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

Osteopontin/OPN is a secretory glycophosphoprotein belonging to the SIBLING family. It contains RGD sequences and phosphorylation sites and can bind to integrins αvβ3, α4β1 and CD44 receptors. Osteopontin/OPN regulates cell adhesion and migration through receptor-mediated regulation, activates MAPK/ERK and PI3K-AKT to promote tumor invasion, and upregulates TIMP-1/2 to inhibit MMPs and protect cartilage. Osteopontin/OPN can be used in the study of osteoarthritis, liver cancer, etc[1][2][3].

研究背景

Osteopontin/OPN 是一种 44-75 kDa 的分泌型糖磷蛋白,属于小整合素结合配体 N-糖蛋白 (SIBLING) 家族,含 RGD (Arg-Gly-Asp) 序列及多个磷酸化位点,可与整合素 αvβ3、α4β1CD44 受体结合[1][2][3]
Osteopontin/OPN 通过受体介导调控细胞黏附、迁移、增殖及基质重塑,参与炎症、骨代谢及肿瘤进展。Osteopontin/OPN 可上调 TIMP-1TIMP-2,抑制基质金属蛋白酶 (MMPs) 活性,保护软骨细胞。Osteopontin/OPN 还通过 NF-κB 通路诱导炎症因子释放,参与骨关节炎 (OA) 病理进程[1]。同时,Osteopontin/OPN 激活 MAPK/ERK 和 PI3K-AKT 通路,促进肿瘤细胞侵袭 (如骨肉瘤 OS-732 细胞迁移)[2]; Osteopontin/OPN 在上游受 TNF-α、IL-1β 诱导,在下游激活整合素-细胞骨架信号级联[3]
Osteopontin/OPN 可用于骨关节炎 (OA) 的研究。关节内 OPN 通过上调 TIMP-1/2 抑制 MMPs,延缓软骨降解[1]。Osteopontin/OPN 还可用于肿瘤诊断与预后的研究。术前血浆 OPN 水平是肝癌 (HCC) 复发和不良预后的独立预测因子 (临界值 200 ng/mL)[3];而反义 OPN RNA 可抑制骨肉瘤细胞致瘤性,延长裸鼠肿瘤潜伏期并减少瘤重[2]

体外研究
(In Vitro)

Osteopontin/OPN (人源;100 ng/mL、1 μg/mL;48 h) 对人膝骨关节炎软骨细胞无细胞毒性;在 1 μg/mL 浓度下,显著上调人膝骨关节炎软骨细胞中 TIMP-1 和 TIMP-2 mRNA 水平,而 100 ng/mL 组无显著变化[1]

体内研究
(In Vivo)

Osteopontin/OPN (人源;反义 RNA 转染;细胞移植;27 天) 在 Balb/c 裸鼠骨肉瘤 (OS-732 细胞) 皮下移植模型中,反义 hOPN RNA 转染的 OS-732 细胞显著延长肿瘤潜伏期至 14 天 (对照组为 7 天),抑制肿瘤生长,降低肿瘤质量[2]

生物活性

Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 10^5 cells/well are added to Recombinant Human Osteopontin coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C. The ED50 for this effect is <0.3 μg/mL.

  • Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 105 cells/well are added to Recombinant Human Osteopontin coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C. The ED50 for this effect is 0.1486 μg/mL, corresponding to a specific activity is 6729.475 units/mg.
种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P10451-1 (I17-N314)

基因 ID
蛋白结构
N-term
OPN (I17-N314)
Accession # P10451-1
6*His
C-term
蛋白长度

Full Length of Isoform-1 Mature Protein

中文名
Osteopontin 蛋白/OPN 蛋白
同用名
Osteopontin; Bone Sialoprotein 1; Nephropontin; Secreted Phosphoprotein 1; SPP-1; Urinary Stone Protein; Uropontin; SPP1; BNSP; OPN
氨基酸序列

IPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSNESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELDSASSEVN

分子量

Approximately 50-69 kDa due to the glycosylation

糖基化修饰
Yes
纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 8% trehalose.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

Osteopontin/OPN Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Osteopontin/OPN Protein, Human (HEK293, His)
目录号:
HY-P70499
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