p38 alpha/MAPK14 蛋白, Human (Activated, sf9, His)

The p38 α/MAPK14 protein is an important serine/threonine kinase in the MAP kinase pathway. It can respond to stimuli such as cytokines or stress and directly activate transcription factors. In this cascade, p38 MAPK each phosphorylates approximately 200 to 300 substrates, including the downstream kinases RPS6KA5/MSK1 and RPS6KA4/MSK2, which are critical for immediate early gene induction. p38 alpha/MAPK14 Protein, Human (Activated, sf9, His) is the recombinant human-derived p38 alpha/MAPK14 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of p38 alpha/MAPK14 Protein, Human (Activated, sf9, His) is 360 a.a., with molecular weight of ~43.7 kDa.

p38 α/MAPK14 是 MAP 激酶信号转导途径不可或缺的丝氨酸/苏氨酸激酶，是细胞对促炎细胞因子或物理应激等细胞外刺激的反应至关重要的四种 p38 MAPK 之一，从而导致转录因子的直接激活。在这个级联中，p38 MAPK 磷酸化多种蛋白质，估计每个蛋白质大约有 200 至 300 个底物。其中包括 RPS6KA5/MSK1 和 RPS6KA4/MSK2 等下游激酶，它们直接磷酸化和激活转录因子和组蛋白 H3，在响应应激或有丝分裂刺激而快速诱导即早期基因中发挥关键作用。此外，p38 MAPK14 调节蛋白质翻译，影响细胞骨架重塑，并参与各种细胞过程，例如内体动力学、蛋白质周转和跨膜蛋白的胞外域脱落。在细胞核中，它磷酸化并激活参与炎症反应的转录因子，调节基因表达和染色质动力学。此外，p38 MAPK14 涉及多种细胞功能，包括葡萄糖摄取、皮肤发育和红细胞生成的调节，并表现出激酶独立的作用，例如 O-Glc-N-酰化的调节。它响应 UV-B 照射和核糖体碰撞而被磷酸化激活，导致 NLRP1 炎性体激活和细胞焦亡。此外，在微生物感染期间，p38 α/MAPK14 被磷酸化激活，抑制 T 细胞中 IFN-γ 的产生。

The specific activity was determined to be > 50 nmol/min/mg using MBP as substrate.

Human

Sf9 insect cells

N-His

Q16539-2/NP_001306.1 (M1-S360)

1432  [NCBI]

Approximately 43.7 kDa

Greater than 85% as determined by reducing SDS-PAGE

溶液.

Supplied as sterile 50 mM Tris-HCl, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol, pH 7.5

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.