PAP 蛋白, Human (His)

PAP proteins play a dual regulatory role in cell growth, exhibiting different effects depending on the specific growth factors involved. In fibroblasts, it exerts a positive regulatory effect by enhancing PDGFA-stimulated cell growth. PAP Protein, Human (His) is the recombinant human-derived PAP protein, expressed by E. coli , with N-6*His labeled tag. The total length of PAP Protein, Human (His) is 181 a.a., with molecular weight of ~30.0 kDa.

PAP 蛋白在细胞生长中发挥双重调节作用，根据所涉及的特定生长因子表现出不同的效果。在成纤维细胞中，它通过增强 PDGFA 刺激的细胞生长发挥正调节作用。然而，同时，它通过抑制 PDGFB 的促有丝分裂作用而充当负调节剂。这种蛋白质对细胞生长发挥微调控制，可能影响细胞对不同生长因子的反应，并有助于细胞增殖的整体平衡和调节。PAP 蛋白与 PDGFA 和 PDGFB 等生长因子之间错综复杂的相互作用凸显了细胞生长调节的复杂性以及了解所涉及的特定分子机制的重要性。

Human

E. coli

N-6*His

Q13442 (M1-K181)

11333  [NCBI]

MPKGGRKGGHKGRARQYTSPEEIDAQLQAEKQKAREEEEQKEGGDGAAGDPKKEKKSLDSDESEDEEDDYQQKRKGVEGLIDIENPNRVAQTTKKVTQLDLDGPKELSRREREEIEKQKAKERYMKMHLAGKTEQAKADLARLAIIRKQREEAARKKEEERKAKDDATLSGKRMQSLSLNK

Approximately 30.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 0.1 mM PMSF, 2 mM DTT, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.