Periostin 蛋白, Mouse (811a.a, HEK293, His)

Periostin protein is a multifunctional molecule that plays a key role in cellular processes by inducing cell attachment and spreading, emphasizing its importance in cell adhesion dynamics. Furthermore, Periostin contributes to the structural integrity of connective tissue by enhancing the binding of BMP1 to the fibronectin matrix and promoting subsequent proteolytic activation of lysyl oxidase LOX. Periostin Protein, Mouse (811a.a, HEK293, His) is the recombinant mouse-derived Periostin protein, expressed by HEK293 , with C-His labeled tag. The total length of Periostin Protein, Mouse (811a.a, HEK293, His) is 811 a.a., with molecular weight of 80-85 kDa.

骨膜素蛋白是一种多功能分子，通过诱导细胞附着和扩散在细胞过程中发挥关键作用，证明了其在细胞粘附动力学中的重要性。此外，Periostin 通过增强 BMP1 与纤连蛋白基质的结合，促进赖氨酰氧化酶 LOX 的随后蛋白水解激活，从而有助于结缔组织的结构完整性。在结构上，Periostin 以同二聚体形式存在，在功能上，它与 BMP1 和纤连蛋白等关键伙伴相互作用。这种复杂的相互作用网络强调了 Periostin 在介导细胞粘附和促进结缔组织细胞外基质结构方面的多种功能，强调了其在维持组织完整性和功能方面的重要作用。

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. Immobilized Recombinant Mouse Periostin at 10 µg/mL (100 µL/well) induces 52.34% cell adhesion.

Mouse

HEK293

C-His

Q62009 (M1-Q811)

50706  [NCBI]

NSYYDKVLAHSRIRGRDQGPNVCALQQILGTKKKYFSSCKNWYQGAICGKKTTVLYECCPGYMRMEGMKGCPAVMPIDHVYGTLGIVGATTTQHYSDVSKLREEIEGKGSYTYFAPSNEAWENLDSDIRRGLENNVNVELLNALHSHMVNKRMLTKDLKHGMVIPSMYNNLGLFINHYPNGVVTVNCARVIHGNQIATNGVVHVIDRVLTQIGTSIQDFLEAEDDLSSFRAAAITSDLLESLGRDGHFTLFAPTNEAFEKLPRGVLERIMGDKVASEALMKYHILNTLQCSEAITGGAVFETMEGNTIEIGCEGDSISINGIKMVNKKDIVTKNGVIHLIDEVLIPDSAKQVIELAGKQQTTFTDLVAQLGLASSLKPDGEYTLLAPVNNAFSDDTLSMDQRLLKLILQNHILKVKVGLSDLYNGQILETIGGKQLRVFVYRTAICIENSCMVRGSKQGRNGAIHIFREIIQPAEKSLHDKLRQDKRFSIFLSLLEAADLKDLLTQPGDWTLFAPTNDAFKGMTSEERELLIGDKNALQNIILYHLTPGVYIGKGFEPGVTNILKTTQGSKIYLKGVNETLLVNELKSKESDIMTTNGVIHVVDKLLYPADIPVGNDQLLELLNKLIKYIQIKFVRGSTFKEIPMTVYTTKIITKVVEPKIKVIQGSLQPIIKTEGPAMTKIQIEGDPDFRLIKEGETVTEVIHGEPVIKKYTKIIDGVPVEITEKQTREERIITGPEIKYTRISTGGGETGETLQKFLQKEVSKVTKFIEGGDGHLFEDEEIKRLLQ

80-85 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.