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PGD 蛋白, Human (HEK293, His)

目录号: HY-P7450
COA 产品使用指南

PGD 蛋白, Human (HEK293, His)是一种二聚体蛋白,N 端带有 His 标签。PGD 蛋白, His (HEK293-expressed) 可将 6-磷酸葡萄糖酸酯转化为核糖-5-磷酸。

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规格 价格 是否有货 数量
10 μg ¥750 询价
50 μg ¥2100 In-stock
100 μg   询价  

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描述

PGD Protein, Human (HEK293, His) is a homodimeric protein, with an N-terminal His tag. Recombinant Human PGD, His (HEK293-expressed) is a key enzyme that converts 6-phosphogluconate into ribulose-5-phosphate with NADP+ as cofactor in the pentose phosphate pathway (PPP)[1].

研究背景

PGD 通常上调,并在许多人类癌症中发挥重要作用,但 PGD 发挥这种作用的机制仍然难以捉摸。这种磷酸化通过增加 6PGD 与 NADP+ 的结合亲和力来增强 6PGD 活性,从而激活 NADPH 和 5-磷酸核糖的 PPP,从而解毒细胞内活性氧 (ROS) 并加速 DNA 合成。此外,6PGD pY481与Fyn表达、人胶质母细胞瘤的恶性程度和预后相关。这些发现证实了 Fyn 依赖性 6PGD 磷酸化在 EGF 促进的肿瘤生长和辐射耐受性中的关键作用[1]

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P52209 (M1-A483)

基因 ID

5226  [NCBI]

蛋白结构
N-term
PGD (M1-A483)
Accession # P52209
6*His
C-term
同用名
rHuPGD, His; 6-phosphogluconate dehydrogenase, decarboxylating; PGD
氨基酸序列

MAQADIALIGLAVMGQNLILNMNDHGFVVCAFNRTVSKVDDFLANEAKGTKVVGAQSLKEMVSKLKKPRRIILLVKAGQAVDDFIEKLVPLLDTGDIIIDGGNSEYRDTTRRCRDLKAKGILFVGSGVSGGEEGARYGPSLMPGGNKEAWPHIKTIFQGIAAKVGTGEPCCDWVGDEGAGHFVKMVHNGIEYGDMQLICEAYHLMKDVLGMAQDEMAQAFEDWNKTELDSFLIEITANILKFQDTDGKHLLPKIRDSAGQKGTGKWTAISALEYGVPVTLIGEAVFARCLSSLKDERIQASKKLKGPQKFQFDGDKKSFLEDIRKALYASKIISYAQGFMLLRQAATEFGWTLNYGGIALMWRGGCIIRSVFLGKIKDAFDRNPELQNLLLDDFFKSAVENCQDSWRRAVSTGVQAGIPMPCFTTALSFYDGYRHEMLPASLIQAQRDYFGAHTYELLAKPGQFIHTNWTGHGGTVSSSSYNAHHHHHH

分子量

Approximately 58.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液

组分

Supplied as a 0.2 μm filter solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
参考文献

PGD Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PGD Protein, Human (HEK293, His)
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