PIST 蛋白, Human (His)

PIST (PDZ domain-containing protein that interacts with Stx6) is critical in intracellular protein trafficking and degradation. It modulates CFTR chloride currents and acid-induced ASIC3 currents, thereby potentially regulating their cell surface expression. PIST Protein, Human (His) is the recombinant human-derived PIST protein, expressed by E. coli , with N-His labeled tag. The total length of PIST Protein, Human (His) is 177 a.a., with molecular weight of 25-30 kDa.

PIST（与 Stx6 相互作用的包含 PDZ 结构域的蛋白质）在细胞内蛋白质运输和降解中发挥着至关重要的作用，其参与多种细胞过程就证明了这一点。它参与 CFTR 氯化物电流和酸诱导的 ASIC3 电流的调节，可能调节这两个通道的细胞表面表达。此外，PIST 与 ADR1B 受体的细胞内运输有关，可能有助于自噬调节。PIST 与 MARCHF2 合作，介导 CFTR 的泛素化和溶酶体降解，导致其在细胞内滞留和随后的溶酶体降解。该蛋白质形成同源寡聚物并参与与各种伙伴的相互作用网络，包括FZD5、FZD8、GRID2、BECN1、CSPG5、CLCN3、STX6、CFTR、ASIC3、GOLGA3、NLGN1、RHOQ、MARCHF2、ADRB1以及潜在的CACNG2和CCDC62，强调其在细胞运输和信号传导途径中的多方面作用。

Human

E. coli

N-His

Q9HD26 (I286-Y462)

57120  [NCBI]

IRKVLLLKEDHEGLGISITGGKEHGVPILISEIHPGQPADRCGGLHVGDAILAVNGVNLRDTKHKEAVTILSQQRGEIEFEVVYVAPEVDSDDENVEYEDESGHRYRLYLDELEGGGNPGASCKDTSGEIKVLQGFNKKAVTDTHENGDLGTASETPLDDGASKLDDLHTLYHKKSY

Approximately 25 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.