PLA2G16 蛋白, Human (His)

The PLA2G16 protein has dual functions, acting as a phospholipase to exert calcium-independent PLA1 and PLA2 activities, preferentially releasing fatty acids through PLA1. It also acts as an O-acyltransferase and N-acyltransferase, helping to form N-acylphosphatidylethanolamine (the precursor of N-acylethanolamine). PLA2G16 Protein, Human (His) is the recombinant human-derived PLA2G16 protein, expressed by E. coli , with N-6*His labeled tag. The total length of PLA2G16 Protein, Human (His) is 121 a.a., with molecular weight of 14-17 kDa.

PLA2G16 蛋白具有双重功能，包括磷脂酶 A1/2 和酰基转移酶活性。作为一种磷脂酶，它表现出不依赖于钙的 PLA1 和 PLA2 活性，从甘油磷脂的 sn-1 或 sn-2 位置有效释放脂肪酸，并且具有显着的 PLA1 活性。此外，PLA2G16 作为 O-酰基转移酶，促进脂肪酰基从甘油磷脂转移到溶血磷脂的羟基，并作为 N-酰基转移酶，催化脂肪酰基从磷脂酰胆碱和其他甘油磷脂的钙独立转移为磷脂酰乙醇胺的伯胺。该过程形成 N-酰基磷脂酰乙醇胺，它是 N-酰基乙醇胺的前体。除了其脂质修饰酶活性外，PLA2G16 在眼晶状体终末分化过程中的细胞器破裂和降解中发挥着至关重要的作用，有助于晶状体透明度。此外，在微生物感染的情况下，它充当小核糖核酸病毒的宿主因子，促进早期感染期间病毒基因组释放到细胞质中，并充当病毒进入位点膜损伤的细胞传感器。这种多方面的功能强调了 PLA2G16 在细胞和感染相关过程中的不同作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

P53816 (D12-D132)

11145  [NCBI]

DLIEIFRPFYRHWAIYVGDGYVVHLAPPSEVAGAGAASVMSALTDKAIVKKELLYDVAGSDKYQVNNKHDDKYSPLPCSKIIQRAEELVGQEVLYKLTSENCEHFVNELRYGVARSDQVRD

14-17 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.