1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. PLA2G2D 蛋白, Mouse (HEK293, Fc)

PLA2G2D 蛋白, Mouse (HEK293, Fc)

目录号: HY-P76549
COA 产品使用指南

PLA2G2D 蛋白是一种分泌型钙依赖性磷脂酶 A2,靶向细胞外脂质,具有抗炎和免疫抑制功能。它水解sn-2位的脂肪酰基,优先水解磷脂酰乙醇胺和磷脂酰甘油。PLA2G2D 蛋白, Mouse (HEK293, Fc) 是重组的 PLA2G2D 蛋白,由 HEK293 表达,带有 C-hFc 标签。PLA2G2D 蛋白, Mouse (HEK293, Fc) 全长 125 个氨基酸,分子量约为 40-50 kDa。

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描述

PLA2G2D protein is a secreted calcium-dependent phospholipase A2 that targets extracellular lipids and has anti-inflammatory and immunosuppressive functions. It hydrolyzes the fatty acyl group at the sn-2 position, preferentially hydrolyzing phosphatidylethanolamine and phosphatidylglycerol. PLA2G2D Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived PLA2G2D protein, expressed by HEK293 , with C-hFc labeled tag. The total length of PLA2G2D Protein, Mouse (HEK293, Fc) is 125 a.a., with molecular weight of 40-50 kDa.

研究背景

PLA2G2D 蛋白是一种分泌型钙依赖性磷脂酶 A2,主要针对细胞外脂质,发挥抗炎和免疫抑制功能。它水解磷脂 sn-2 位上脂肪酰基的酯键,优先水解磷脂酰乙醇胺和磷脂酰甘油而不是磷脂酰胆碱。在引流淋巴结中,它选择性地水解二酰基和烯基形式的磷脂酰乙醇胺,释放 omega-3 多不饱和脂肪酸 (PUFA),例如二十碳五烯酸和二十二碳六烯酸,它们是称为分解素的抗炎脂质介质的前体。在急性炎症的消退阶段,PLA2G2D 蛋白驱动二十二碳六烯酸衍生的消退素 D1 的合成,抑制树突状细胞活化和 T 辅助细胞 1 免疫反应。除了其催化活性外,PLA2G2D 蛋白还可促进调节性 T 细胞 (Treg) 的分化并参与免疫耐受的维持。它还可能有助于细胞膜的脂质重塑和参与病原体清除的脂质介质的产生。此外,PLA2G2D 蛋白通过直接水解细菌膜的磷脂,对革兰氏阳性菌具有杀菌活性。

种属

Mouse

表达系统

HEK293

标签

C-hFc

蛋白编号

Q9WVF6-1 (G20-C144)

基因 ID

18782  [NCBI]

蛋白结构
N-term
PLA2G2D (G20-C144)
Accession # Q9WVF6
hFc
C-term
同用名
Group IID secretory phospholipase A2; GIID sPLA2; PLA2IID; Splash
氨基酸序列

GLLNLNKMVTHMTGKKAFFSYWPYGCHCGLGGKGQPKDATDWCCQKHDCCYAHLKIDGCKSLTDNYKYSISQGTIQCSDNGSWCERQLCACDKEVALCLKQNLDSYNKRLRYYWRPRCKGKTPAC

分子量

Approximately 40-50 kDa due to the glycosylation.

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PLA2G2D Protein, Mouse (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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