PLA2G7 蛋白, Mouse (419a.a, HEK293, His)

The PLA2G7 protein is a lipoprotein-associated calcium-independent phospholipase A2 that plays a key role in phospholipid catabolism during inflammation and oxidative stress responses. It acts at the lipid-water interface and hydrolyzes the ester bond of the fatty acyl group at the sn-2 position, with particular preference for short-chain fatty acyl groups. PLA2G7 Protein, Mouse (419a.a, HEK293, His) is the recombinant mouse-derived PLA2G7 protein, expressed by HEK293 , with C-His, C-6*His labeled tag. The total length of PLA2G7 Protein, Mouse (419a.a, HEK293, His) is 419 a.a., with molecular weight of ~52-65 KDa.

PLA2G7 蛋白是一种脂蛋白相关的钙非依赖性磷脂酶 A2，在炎症和氧化应激反应过程中的磷脂分解代谢中发挥着关键作用。它在脂质-水界面作用，水解磷脂 sn-2 位脂肪酰基的酯键，特别优选那些带有短链脂肪酰基的酯键。此外，PLA2G7 可以靶向具有长脂肪酰基链的磷脂（如果它们带有氧化官能团）。该酶的多功能性延伸至灭活血小板激活因子（PAF）（一种有效的促炎信号脂质），以及水解氧化截短的磷脂，防止其积累和不受控制的促炎作用。当与高密度脂蛋白 (HDL) 颗粒结合时，PLA2G7 有助于水解含有长链脂肪酰基氢过氧化物的磷脂，防止潜在的氧脂质在血管壁中积聚。此外，PLA2G7 催化 F2-异前列腺素的释放，作为细胞氧化损伤的脂质生物标志物。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

HEK293

C-His;C-6*His

Q60963/NP_038765.2(F22-N440)

27226  [NCBI]

FHWQDTSSFDFRPSVMFHKLQSVMSAAGSGHSKIPKGNGSYPVGCTDLMFGYGNESVFVRLYYPAQDQGRLDTVWIPNKEYFLGLSIFLGTPSIVGNILHLLYGSLTTPASWNSPLRTGEKYPLIVFSHGLGAFRTIYSAIGIGLASNGFIVATVEHRDRSASATYFFEDQVAAKVENRSWLYLRKVKQEESESVRKEQVQQRAIECSRALSAILDIEHGDPKENVLGSAFDMKQLKDAIDETKIALMGHSFGGATVLQALSEDQRFRCGVALDPWMYPVNEELYSRTLQPLLFINSAKFQTPKDIAKMKKFYQPDKERKMITIKGSVHQNFDDFTFVTGKIIGNKLTLKGEIDSRVAIDLTNKASMAFLQKHLGLQKDFDQWDPLVEGDDENLIPGSPFDAVTQVPAQQHSPGSQTQN

Approximately 52-65 kDa.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.