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  4. PLAU/uPA 蛋白, Rat (HEK293, His)

PLAU/uPA 蛋白, Rat (HEK293, His)

目录号: HY-P73561
COA 产品使用指南

PLAU/uPA 蛋白的特点是缺乏对特征注释传播至关重要的保守残基,这引发了有关其结构和功能方面的有趣问题。这种独特性表明纤溶酶原激活和纤维蛋白溶解调节中分子相互作用的潜在差异。PLAU/uPA 蛋白, Rat (HEK293, His) 是重组的 PLAU/uPA 蛋白,由 HEK293 表达,带有 C-His 标签。PLAU/uPA 蛋白, Rat (HEK293, His) 全长 413 个氨基酸,分子量为 30-35 kDa。

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描述

PLAU/uPA proteins are characterized by a lack of conserved residues critical for feature annotation propagation, raising interesting questions about their structural and functional aspects. This uniqueness suggests potential differences in molecular interactions in plasminogen activation and fibrinolysis regulation. PLAU/uPA Protein, Rat (HEK293, His) is the recombinant rat-derived PLAU/uPA protein, expressed by HEK293 , with C-His labeled tag. The total length of PLAU/uPA Protein, Rat (HEK293, His) is 413 a.a., with molecular weight of 30-35 kDa.

研究背景

PLAU,也称为 uPA(尿激酶型纤溶酶原激活剂),其特点是不存在特征注释传播所需的保守残基。这一独特的特征引发了有关 PLAU 结构和功能方面的有趣问题,表明其在纤溶酶原激活和纤维蛋白溶解调节背景下分子相互作用的潜在区别。这些保守残基的缺失强调了 PLAU 的特定属性,并强调需要进行深入研究以阐明其在细胞过程和病理条件中的独特作用。

生物活性

Human uPAR, hFc Tag captured on CM5 Chip via Protein A can bind Rat PLAU, His Tag with an affinity constant of 4.31 μM as determined in SPR assay (Biacore T200).

种属

Rat

表达系统

HEK293

标签

C-His

蛋白编号

Q3KR76/NP_037217.3 (G20-F432)

基因 ID

25619  [NCBI]

蛋白结构
N-term
PLAU (G20-F432)
Accession # Q3KR76/NP_037217.3
His
C-term
同用名
Urokinase-type plasminogen activator; uPA; PLAU
分子量

30-35 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

PLAU/uPA Protein, Rat (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PLAU/uPA Protein, Rat (HEK293, His)
目录号:
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