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PPA1 蛋白, S. cerevisiae (His)

目录号: HY-P79310
COA 产品使用指南

PPA1 蛋白是 PPase 家族的重要成员,在细胞过程中发挥着重要作用,特别是与磷酸盐代谢相关的去磷酸化。PPA1 与相关蛋白质具有保守的特征,参与磷酸酶活性。它在 PPase 家族中的分类强调了其独特的酶功能。研究 PPA1 有助于了解其作用和潜在的治疗应用,并深入了解其对细胞过程的更广泛影响。进一步的探索有望增强人们对 PPA1 对正常生理和病理状况的贡献的了解。PPA1 蛋白, S. cerevisiae (His) 是重组的 PPA1 蛋白,由 E. coli 表达,不带标签。PPA1 蛋白, S. cerevisiae (His) 全长 287 个氨基酸,分子量约为 ~34.19 kDa。

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规格 价格 是否有货 数量
50 μg ¥1600 In-stock
500 μg ¥7600 In-stock
> 100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

PPA1 Protein, a crucial member of the PPase family, plays a significant role in cellular processes, particularly dephosphorylation related to phosphate metabolism. Sharing conserved features with related proteins, PPA1 is involved in phosphatase activities. Its classification within the PPase family emphasizes its unique enzymatic functions. Studying PPA1 contributes to understanding its role and potential therapeutic applications, providing insights into its broader impact on cellular processes. Further exploration promises to enhance knowledge of PPA1's contributions to both normal physiology and pathological conditions. PPA1 Protein, S. cerevisiae (His) is the recombinant human-derived PPA1 protein, expressed by E. coli, with tag free. The total length of PPA1 Protein, S. cerevisiae (His) is 287 a.a., with molecular weight of ~34.19 kDa.

研究背景

PPA1 蛋白是 PPase 家族的重要成员,凸显了其在细胞过程中的重要作用。作为该酶家族的一部分,PPA1 可能与相关蛋白质具有保守的结构和功能特征,这表明它参与磷酸酶活性。PPase 家族的分类强调了其在更广泛的磷酸酶背景下的特定名称,提供了对其独特酶功能的深入了解。PPA1 的研究有助于我们了解其在与去磷酸化相关的细胞过程中的作用,提供在治疗干预中的潜在应用,并更深入地理解其对参与磷酸盐代谢的细胞过程的更广泛影响。对 PPA1 作用的进一步探索有望增强我们对其对正常生理和病理状况的贡献的了解。

生物活性

Measured by its ability to hydrolyze pyrophosphate. The specific activity is 167023 pmol/min/μg, as measured under the described conditions.

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

A6ZKV8 (M1-V287)

基因 ID

852296  [NCBI]

蛋白结构
N-term
PPA1 (M1-V287)
Accession # A6ZKV8
C-term
同用名
Inorganic pyrophosphatase; IPP1; Pyrophosphate phospho-hydrolase; PPase; PPA; PPA1
氨基酸序列

MTYTTRQIGAKNTLEYKVYIEKDGKPVSAFHDIPLYADKENNIFNMVVEIPRWTNAKLEITKEETLNPIIQDTKKGKLRFVRNCFPHHGYIHNYGAFPQTWEDPNVSHPETKAVGDNDPIDVLEIGETIAYTGQVKQVKALGIMALLDEGETDWKVIAIDINDPLAPKLNDIEDVEKYFPGLLRATNEWFRIYKIPDGKPENQFAFSGEAKNKKYALDIIKETHDSWKQLIAGKSSDSKGIDLTNVTLPDTPTYSKAASDAIPPASPKADAPIDKSIDKWFFISGSV

分子量

Approximately 34.19 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE
性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 10 mM Tris, 50 mM NaCl, 1 mM DDT, 0.1 mM EDTA, 50% Glycerol, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

PPA1 Protein, S. cerevisiae (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PPA1 Protein, S. cerevisiae (His)
目录号:
HY-P79310
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