蛋白 E6, HPV16 (His)

Protein E6 is one of three cancer proteins encoded by human papillomavirus (HPV). The binding of Protein E6 to ubiquitin protein ligase can inhibit the expression of p53. Protein E6 suppresses the immune response through the JAK-STAT signaling pathway. Protein E6 can promote cell proliferation and inhibit cell apoptosis. Protein E6, HPV16 (His) is the recombinant Virus-derived protein E6, HPV16, expressed by E. coli , with N-6*His labeled tag. The total length of Protein E6, HPV16 (His) is 158 a.a., the molecular weight are 22 KDa (monomer), 44 KDa (dimer), whiel dimers are generally formed.

Protein E6 是一种癌蛋白，通过刺激许多宿主细胞关键调节蛋白在细胞转化的诱导和维持中起主要作用。Protein E6 与宿主UBE3A/E6-AP 泛素蛋白连接酶结合，通过靶向26S 蛋白酶体降解肿瘤抑制因子TP53 和TP73 ，使其失活。反过来，DNA 损伤和染色体不稳定性增加，导致细胞增殖和癌症发展。Protein E6 可形成复合物E6/E6AP 降解包括BAK1、Fas 相关死亡结构域蛋白(FADD) 和procaspase 8 抑制细胞凋亡。Protein E6 还通过与宿主IRF3 和TYK2 相互作用抑制免疫应答。这些相互作用阻止IRF3 转录活性，抑制干扰素α 介导的TYK2 介导的JAK-STAT 激活，从而抑制干扰素信号通路。Protein E6 可激活早期传代人角质形成细胞和乳腺上皮细胞的端粒酶。Protein E6 与hDLG 或其他含有PDZ 结构域的蛋白之间的相互作用可能是HPV 相关癌症发展的潜在机制。

Measured in a cell proliferation assay using A549 cells. The ED₅₀  for this effect is <6.8 ng/mL, corresponding to a specific activity is >1.47×10⁵ units/mg.

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  Measured in a cell proliferation assay using A549 cells. The ED₅₀  for this effect is 3.586 ng/mL, corresponding to a specific activity is 2.79×10⁵ units/mg.

Virus

E. coli

N-6*His

P03126 (M1-L158)

1489078  [NCBI]

MHQKRTAMFQDPQERPRKLPQLCTELQTTIHDIILECVYCKQQLLRREVYDFAFRDLCIVYRDGNPYAVCDKCLKFYSKISEYRHYCYSLYGTTLEQQYNKPLCDLLIRCINCQKPLCPEEKQRHLDKKQRFHNIRGRWTGRCMSCCRSSRTRRETQL

Monomer: 22 kDa Dimer: 44 kDa. It is speculated that the protein forms a dimeric structure.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from 0.22 μm filtered solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCl, 500 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.