Prothrombin 蛋白, Human (HEK293, His)

Prothrombin or factor II is a key protein in a variety of physiological processes. The serine protease thrombin, derived from prothrombin, cleaves the bond behind arginine and lysine residues, converting fibrinogen to fibrin. Prothrombin Protein, Human (HEK293, His) is the recombinant human-derived Prothrombin protein, expressed by HEK293 , with C-10*His labeled tag. The total length of Prothrombin Protein, Human (HEK293, His) is 622 a.a., with molecular weight of ~75-100 kDa.

凝血酶原蛋白，也称为因子 II，在各种生理过程中发挥着关键作用。凝血酶是一种源自凝血酶原的丝氨酸蛋白酶，可切割精氨酸和赖氨酸残基后的键，促进纤维蛋白原转化为纤维蛋白。此外，凝血酶原积极参与 V、VII、VIII、XIII 等关键因子的激活，当与血栓调节蛋白、蛋白 C 结合使用时，凝血酶原除了在血液稳态中发挥核心作用外，还对炎症和复杂的炎症过程有显着影响。伤口愈合。

Human

HEK293

C-10*His

P00734 (M1-E622)

2147

MAHVRGLQLPGCLALAALCSLVHSQHVFLAPQQARSLLQRVRRANTFLEEVRKGNLERECVEETCSYEEAFEALESSTATDVFWAKYTACETARTPRDKLAACLEGNCAEGLGTNYRGHVNITRSGIECQLWRSRYPHKPEINSTTHPGADLQENFCRNPDSSTTGPWCYTTDPTVRRQECSIPVCGQDQVTVAMTPRSEGSSVNLSPPLEQCVPDRGQQYQGRLAVTTHGLPCLAWASAQAKALSKHQDFNSAVQLVENFCRNPDGDEEGAWCYVAGKPGDFGYCDLNYCEEAVEEETGDGLDEDSDRAIEGRTATSEYQTFFNPRTFGSGEADCGLRPLFEKKSLEDKTERELLESYIDGRIVEGSDAEIGMSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYERNIEKISMLEKIYIHPRYNWRENLDRDIALMKLKKPVAFSDYIHPVCLPDRETAASLLQAGYKGRVTGWGNLKETWTANVGKGQPSVLQVVNLPIVERPVCKDSTRIRITDNMFCAGYKPDEGKRGDACEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDQFGE

Approximately 75-100 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from sterile 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.