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  4. PSMG4 蛋白, Human (His, Strep)

PSMG4 蛋白, Human (His, Strep)

目录号: HY-P701391
产品使用指南

PSMG4 蛋白作为伴侣，促进 20S 蛋白酶体的组装。作为伴侣，PSMG4 与 PSMG3 相互作用，形成功能复合物，有助于 20S 蛋白酶体的正确组装。值得注意的是，PSMG4 与 20S 蛋白酶体的 α 亚基特异性相关，突显其参与蛋白酶体成熟和功能的复杂过程。PSMG4 蛋白, Human (His, Strep) 是重组的 PSMG4 蛋白，由 E. coli 表达，带有 N-Strep, N-6*His 标签。PSMG4 蛋白, Human (His, Strep) 全长 123 个氨基酸。

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描述

PSMG4 protein functions as a chaperone, facilitating the assembly of the 20S proteasome. In its role as a chaperone, PSMG4 interacts with PSMG3, forming a functional complex that contributes to the proper assembly of the 20S proteasome. Notably, PSMG4 associates specifically with the alpha subunits of the 20S proteasome, highlighting its involvement in the intricate process of proteasomal maturation and functionality. PSMG4 Protein, Human (His, Strep) is the recombinant human-derived PSMG4 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of PSMG4 Protein, Human (His, Strep) is 123 a.a., .
研究背景

PSMG4（Proteasome assembly chaperone 4）是一种伴侣蛋白，在促进 20S 蛋白酶体的组装中发挥着至关重要的作用。PSMG4 与 PSMG3 协同作用，促进 20S 蛋白酶体的正确形成和成熟，20S 蛋白酶体是细胞蛋白质降解机制的重要组成部分。值得注意的是，PSMG4 与 20S 蛋白酶体的 α 亚基相互作用，表明它参与协调这种多亚基复合物的组装。PSMG4 的伴侣活性强调了其在确保 20S 蛋白酶体正常功能和结构完整性方面的重要性，有助于细胞调节蛋白质周转和维持细胞稳态的能力。它必须强调 PSMG4 作为伴侣蛋白在促进 20S 蛋白酶体组装及其与 PSMG3 和蛋白酶体 α 亚基的相互作用中的作用。
种属

Human
表达系统

E. coli
标签

N-Strep;N-6*His
蛋白编号

Q5JS54 (M1-F123)
基因 ID

389362
蛋白结构
N-term
Strep-6*His
PSMG4 (M1-F123)
Accession # Q5JS54
C-term
同用名
PSMG4; Proteasome assembly chaperone 4; PAC-4; hPAC4
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

PSMG4 Protein, Human (His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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