PTPN12 蛋白, Human (sf9)

The PTPN12 protein is a multifunctional enzyme that can dephosphorylate a variety of proteins and regulate cell signaling cascades. According to literature, PTPN12 selectively targets tyrosine kinases such as ERBB2 and PTK2B/PYK2 to control related pathways. PTPN12 Protein, Human (sf9) is the recombinant human-derived PTPN12 protein, expressed by Sf9 insect cells, with tag free.

正如文献中记载的那样，PTPN12 蛋白作为一种多功能酶，能够使一系列蛋白质去磷酸化，从而在各种细胞信号级联的调节中发挥着至关重要的作用。在其底物中，PTPN12 选择性地使细胞酪氨酸激酶（包括 ERBB2 和 PTK2B/PYK2）去磷酸化，对涉及这些激酶的信号通路发挥调节控制。PTPN12 的特异性通过其在特定酪氨酸残基（例如“Tyr-1112”、“Tyr-1196”和/或“Tyr-1248”）处使 ERBB2 去磷酸化的能力而凸显。PTPN12 复杂的去磷酸化活性强调了其在调节关键信号传导事件和微调酪氨酸激酶途径介导的细胞反应中的重要性。

Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site.The specific activity is >20 µmol/min/mg.

Human

Sf9 insect cells

Tag Free

Q05209 (N-G&P, M1-Q355)

5782  [NCBI]

Full Length

Approximately 41 kDa

Greater than 85% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.