QPCT 蛋白, Mouse (HEK293, His)

The QPCT protein plays a key role in pyroglutamyl peptide biosynthesis and exhibits substrate preference for N-terminal glutamine residues while showing specificity for residues near the N-terminus. Notably, its catalytic activity is independent of chain length beyond the second residue, emphasizing the importance of QPCT in the precise biosynthesis of pyroglutamyl peptides with diverse N-termini, contributing to various biological background. QPCT Protein, Mouse (HEK293, His) is the recombinant mouse-derived QPCT protein, expressed by HEK293 , with N-His, N-Myc labeled tag. The total length of QPCT Protein, Mouse (HEK293, His) is 327 a.a., with molecular weight of ~41.6 kDa.

QPCT 蛋白在焦谷氨酰肽的生物合成中发挥着关键作用，表现出对具有 N 末端谷氨酰胺残基的底物的偏好，同时对相邻的酸性和色氨酸残基表现出偏好。值得注意的是，其底物特异性对于靠近 N 末端谷氨酰胺残基的残基更为明显，显示出独特的底物识别模式。此外，QPCT 表明对第二个残基以外的链长度没有显着依赖性，这表明该蛋白质的催化活性受超出该点的延伸的影响较小。这种底物识别的选择性和灵活性凸显了 QPCT 在焦谷氨酰肽精确生物合成中的重要性，有助于在各种生物环境中产生具有焦谷氨酰化 N 末端的多种肽。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

HEK293

N-His;N-Myc

Q9CYK2 (36A-362L)

70536  [NCBI]

AWTQEKNHHQPAHLNSSSLQQVAEGTSISEMWQNDLRPLLIERYPGSPGSYSARQHIMQRIQRLQAEWVVEVDTFLSRTPYGYRSFSNIISTLNPEAKRHLVLACHYDSKYFPRWDSRVFVGATDSAVPCAMMLELARALDKKLHSLKDVSGSKPDLSLRLIFFDGEEAFHHWSPQDSLYGSRHLAQKMASSPHPPGSRGTNQLDGMDLLVLLDLIGAANPTFPNFFPKTTRWFNRLQAIEKELYELGLLKDHSLERKYFQNFGYGNIIQDDHIPFLRKGVPVLHLIASPFPEVWHTMDDNEENLHASTIDNLNKIIQVFVLEYLHL

Approximately 41.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.