RAB2 蛋白, Mouse (His)

Rab2A belongs to the Rab family, it is a small molecular weight guanosine triphosphatases (GTPases) and membrane-bound protein which involved in GTP binding and hydrolysis and are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes, Rab2A is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex as well. Rab2A also regulates the compacted morphology of the Golgi. RAB2 Protein, Mouse (His) is the recombinant mouse-derived RAB2 protein, expressed by E. coli , with N-His labeled tag.

Rab2A 属于 Rab 家族，该家族成员是小分子量鸟苷三磷酸酶 (GTPases)，其包含高度保守的结构域，参与 GTP 的结合和水解，是细胞膜内运输从运输囊泡的形成到与膜融合的关键调节剂。Rab2A 是一种膜结合蛋白，是前高尔基中间体的居民，是蛋白质从内质网 (ER) 运输到高尔基复合体所必需的。Rabs 在活跃的 GTP 结合状态和不活跃的 GDP 结合状态之间循环。在它们的活性状态下，驱动囊泡载体从供体细胞器向受体细胞器的运输，以调节维持细胞器身份和形态的膜运输。Rab2A 也调节高尔基体的紧致形态^[1][2]。

Measured in a cell proliferation assay using Hela cells. The ED₅₀ this effect is 2.70 ng/mL, corresponding to a specific activity is 3.70×10⁵ units/mg.

Mouse

E. coli

N-His

Q0PD65 (M1-C212)

59021  [NCBI]

Full Length

MAYAYLFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMITIDGKQIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRDTFNHLTTWLEDARQHSNSNMVIMLIGNKSDLESRREVKKEEGEAFAREHGLIFMETSAKTASNVEEAFINTAKEIYEKIQEGVFDINNEANGIKIGPQHAATNASHGSNQGGQQAGGGCC

Approximately 23-25 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.