RAD51 蛋白, Human (His)

RAD51 Protein, Human (His) is the recombinant human-derived RAD51, expressed by E. coli , with N-6*His labeled tag. ,

Rad51 在 homologous strand exchange 中发挥重要作用，这是通过 homologous recombination (HR) 进行 DNA 修复的关键步骤。它以 ATP 依赖的方式结合 single-stranded DNA，形成 nucleoprotein filaments，这对于 homology search 和 strand exchange 至关重要。Rad51 催化 homologous DNA partners 之间的 homology 识别和 strand exchange，从而在断裂的 DNA 和修复模板之间形成 joint molecule。在 replication stress 期间，Rad51 被招募以解决 stalled replication forks。此外，Rad51 是 PALB2-scaffolded HR 复合物的一部分，该复合物包含 BRCA2 和 RAD51C，被认为在通过 HR 进行 DNA 修复中发挥作用。在 oxidative stress 条件下，Rad51 与 RAD51C 和 XRCC3 一起参与调节 mitochondrial DNA 的拷贝数。Rad51 还参与 interstrand cross-link repair。

Human

E. coli

N-6*His

Q06609-1 (A2-D339)

5888

Full Length of Isoform-1 Mature Protein

42 kDa

Greater than 90 % as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.