NRG1-beta 1 蛋白, Human

NRG-1 Protein, Human belongs to a family of structurally-related polypeptide growth factors derived from alternatively spliced genes, induces Fn14 expression in MCF7 cells.

HRG1-β1 激活亲本 MCF7 细胞中的 HER2/HER3 信号传导并上调 Fn14 基因表达。MCF7/HER2-18 细胞中 HRG1-β1 介导的 Fn14 上调是最大程度产生 MMP-9 所必需的^[1]。

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED₅₀ this effect is ≤ 2.158 ng/mL, corresponding to a specific activity is ≥ 4.6339×10⁵ units/mg.

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  Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED₅₀ for this effect is 2.158 ng/mL, corresponding to a specific activity is 4.6339×10⁵ units/mg.

Human

E. coli

Tag Free

Q02297-6 (S177-E241)

3084  [NCBI]

SHLVKCAEKEKTFCVNGGECFMVKDLSNPSRYLCKCPNEFTGDRCQNYVMASFYKHLGIEFMEAE

Approximately 8 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from sterile PBS or 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Asrani K, et al. The HER2- and heregulin β1 (HRG)-inducible TNFR superfamily member Fn14 promotes HRG-driven breast cancer cell migration, invasion, and MMP9 expression. Mol Cancer Res. 2013 Apr;11(4):393-404.  [Content Brief]