Ribonuclease UK114/HRSP12 蛋白, Human (His)

The ribonuclease UK114/HRSP12 protein catalyzes the hydrolytic deamination of enamine/imine intermediates, reducing the potential toxicity of these reactive metabolites by releasing ammonia. Ribonuclease UK114/HRSP12 Protein, Human (His) is the recombinant human-derived Ribonuclease UK114/HRSP12 protein, expressed by E. coli , with N-6*His labeled tag. The total length of Ribonuclease UK114/HRSP12 Protein, Human (His) is 137 a.a., with molecular weight of 14-16 kDa.

核糖核酸酶 UK114/HRSP12 蛋白通过催化正常代谢过程中产生的烯胺/亚胺中间体的水解脱氨作用，在细胞代谢中发挥至关重要的作用。这种酶活性被认为有助于从这些潜在有毒的反应性代谢物中安全释放氨，从而减轻它们对细胞成分的影响。HRSP12 特别与由各种吡哆醛-5'-磷酸依赖性脱水酶（包括 L-苏氨酸脱水酶）形成的烯胺/亚胺中间体相关。此外，它还通过促进核糖核酸酶 P/MRP 复合物的募集来促进特定转录本的核糖核酸内切裂解。作为 YTHDF2 和核糖核酸酶 P/MRP 复合物之间的桥梁，HRSP12 与含有 N6-甲基腺苷 (m6A) 的 mRNA 结合，该 mRNA 具有 5'-GGUUC-3' 基序。HRSP12 和 YTHDF2 与此类转录物的协同结合导致核糖核酸酶 P/MRP 复合物的募集，从而促进随后的内切核糖核酸切割。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

P52758 (M1-L137)

10247  [NCBI]

MSSLIRRVISTAKAPGAIGPYSQAVLVDRTIYISGQIGMDPSSGQLVSGGVAEEAKQALKNMGEILKAAGCDFTNVVKTTVLLADINDFNTVNEIYKQYFKSNFPARAAYQVAALPKGSRIEIEAVAIQGPLTTASL

14-16 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.