RIPK3 蛋白, Human (P.pastoris, His)

RIPK3 is a kinase that controls necroptosis and apoptosis. In necroptosis, activated by TNF-α and ZBP1, RIPK3 phosphorylates MLKL, leading to membrane damage. RIPK3 Protein, Human (P.pastoris, His) is the recombinant human-derived RIPK3 protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of RIPK3 Protein, Human (P.pastoris, His) is 518 a.a., with molecular weight of ~64 kDa.

RIPK3 是一种丝氨酸/苏氨酸蛋白激酶，可协调坏死性凋亡和细胞凋亡，这是两种不同形式的程序性细胞死亡。坏死性凋亡由诱导死亡的 TNF-α 家族成员触发并由 ZBP1 激活，涉及 RIPK3 介导的 MLKL 磷酸化，导致膜损伤和钙内流。此外，为了应对正粘病毒感染，ZBP1 激活促使核 RIPK3 磷酸化 MLKL，导致核膜破坏和 DNA 泄漏。RIPK3 还依赖于 RIPK1、FADD 和 CASP8 调节细胞凋亡，与 MLKL 和 RIPK3 激酶活性无关。在某些细胞类型中，RIPK3 通过细胞死亡无关的反应来限制病毒复制。在寨卡病毒感染神经元的情况下，RIPK3 与 ZBP1 一起促进不依赖于死亡的转录程序，上调 ACOD1/IRG1 和衣康酸的产生，抑制琥珀酸脱氢酶，并抑制病毒基因组复制。RIPK3 增强代谢酶（GLUL、GLUD1、PYGL）的活性，可能影响三羧酸循环和氧化磷酸化。在单纯疱疹病毒 1 感染期间，RIPK3 与 HHV-1 蛋白 RIR1/ICP6 形成异聚淀粉样蛋白结构，可能抑制 RIPK3 介导的坏死性凋亡，从而使病毒能够逃避宿主细胞死亡途径。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

P. pastoris

N-6*His

Q9Y572 (M1-K518)

11035  [NCBI]

MSCVKLWPSGAPAPLVSIEELENQELVGKGGFGTVFRAQHRKWGYDVAVKIVNSKAISREVKAMASLDNEFVLRLEGVIEKVNWDQDPKPALVTKFMENGSLSGLLQSQCPRPWPLLCRLLKEVVLGMFYLHDQNPVLLHRDLKPSNVLLDPELHVKLADFGLSTFQGGSQSGTGSGEPGGTLGYLAPELFVNVNRKASTASDVYSFGILMWAVLAGREVELPTEPSLVYEAVCNRQNRPSLAELPQAGPETPGLEGLKELMQLCWSSEPKDRPSFQECLPKTDEVFQMVENNMNAAVSTVKDFLSQLRSSNRRFSIPESGQGGTEMDGFRRTIENQHSRNDVMVSEWLNKLNLEEPPSSVPKKCPSLTKRSRAQEEQVPQAWTAGTSSDSMAQPPQTPETSTFRNQMPSPTSTGTPSPGPRGNQGAERQGMNWSCRTPEPNPVTGRPLVNIYNCSGVQVGDNNYLTMQQTTALPTWGLAPSGKGRGLQHPPPVGSQEGPKDPEAWSRPQGWYNHSGK

Approximately 64 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.