1. 重组蛋白
  2. Ubiquitin Related Proteins
  3. Ubiquitin Enzymes
  4. E3 Ligases
  5. Ring Finger Protein 43
  6. RNF43 蛋白, Human (HEK293, Fc)

RNF43 蛋白, Human (HEK293, Fc)

目录号: HY-P76007
产品使用指南

RNF43 是一种 E3 泛素蛋白连接酶,也是 Wnt 信号通路中的关键负调节因子。其作用涉及介导 Wnt 受体复合物(尤其是卷曲蛋白)内成分的泛素化、内吞作用和随后的降解。RNF43 蛋白, Human (HEK293, Fc) 是重组的 RNF43 蛋白,由 HEK293 表达,带有 C-hFc 标签。RNF43 蛋白, Human (HEK293, Fc) 全长 174 个氨基酸,分子量约为 ~52 kDa。

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描述

RNF43 is an E3 ubiquitin protein ligase and a key negative regulator in the Wnt signaling pathway. Its role involves mediating the ubiquitination, endocytosis, and subsequent degradation of components within the Wnt receptor complex, particularly Frizzled. RNF43 Protein, Human (HEK293, Fc) is the recombinant human-derived RNF43 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of RNF43 Protein, Human (HEK293, Fc) is 174 a.a., with molecular weight of ~52 kDa.

研究背景

RNF43 是一种 E3 泛素蛋白连接酶,通过协调 Wnt 受体复合物中关键成分的泛素化、内吞作用和随后的降解,充当 Wnt 信号通路的关键负调节因子,特别针对 Frizzled。这种调节活动延伸到经典和非经典 Wnt 信号通路。RNF43 的作用强调了它作为主开关的一部分,与 RSPO2 和 ZNRF3 一起管理肢体规范。这意味着它在发育过程中的重要性,并强调了它在调节 Wnt 信号传导中的多方面参与,使其成为细胞和发育途径中的关键参与者。

种属

Human

表达系统

HEK293

标签

C-hFc

蛋白编号

Q68DV7 (G24-Y197)

基因 ID
蛋白结构
N-term
RNF43 (G24-Y197)
Accession # Q68DV7
hFc
C-term
同用名
E3 ubiquitin-protein ligase RNF43; RING finger protein 43; RNF43
氨基酸序列

GFGRTGLVLAAAVESERSAEQKAIIRVIPLKMDPTGKLNLTLEGVFAGVAEITPAEGKLMQSHPLYLCNASDDDNLEPGFISIVKLESPRRAPRPCLSLASKARMAGERGASAVLFDITEDRAAAEQLQQPLGLTWPVVLIWGNDAEKLMEFVYKNQKAHVRIELKEPPAWPDY

分子量

Approximately 52 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

RNF43 Protein, Human (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
RNF43 Protein, Human (HEK293, Fc)
目录号:
HY-P76007
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