RuvB 蛋白, E.coli (His-SUMO)

RuvB protein is an important component of the RuvABC complex and plays a key role in recombinant DNA repair, especially for UV-induced damage. It helps rescue stalled DNA replication forks through replication fork reversal (RFR). RuvB Protein, E.coli (His-SUMO) is the recombinant E. coli-derived RuvB protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of RuvB Protein, E.coli (His-SUMO) is 336 a.a., with molecular weight of ~53.2 kDa.

RuvB 蛋白是 RuvABC 复合物的重要组成部分，在紫外线辐射或化学物质损伤的 DNA 重组修复中发挥着关键作用。此外，它还通过复制叉逆转 (RFR)（紫外线生存的重要过程）来拯救停滞的 DNA 复制叉。该亚基表现出弱的 ATP 酶活性，优先与 ADP 结合而不是 ATP，并且其 ATP 酶功能被其 ADP 产物抑制。RuvB 与 RuvA 合作，促进霍利迪连接体 (HJ) 分支迁移，在 ATP 和 Mg(2+) 存在的情况下促进连接点的解离。作为分子泵，RuvB 六聚体将 DNA 拉入并通过 RuvAB 复合物，从而绕过紫外线引起的损伤和物理交联的 DNA 链。RuvA 通过与十字形连接结合提供特异性，而 RuvB ATP 酶则充当分支迁移的动力。RuvA-RuvB 复合物在体外表现出 5'-3' 解旋酶活性，依赖于 ATP，并且对短 dsDNA 杂交体最有效。RuvA 和 RuvB 之间复杂的相互作用与 RuvC 成分一起协调霍利迪连接体的解析并促进分支迁移，突出了 RuvB 蛋白在 DNA 修复和复制过程中的多方面作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

E.coli

E. coli

N-SUMO;N-6*His

P0A812 (M1-P336)

946371  [NCBI]

MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGATTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPEMP

Approximately 53.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.