S100A4 蛋白, Human (C/N-His)

S100A4 protein, a calcium-binding molecule, plays a versatile role in numerous cellular processes, including motility, angiogenesis, cell differentiation, apoptosis, and autophagy. Functionally, it enhances cell motility and invasiveness by interacting with non-muscle myosin heavy chain IIA/MYH9, promoting filament depolymerization and increasing the amount of soluble myosin-IIA to facilitate chemotaxis. Additionally, S100A4 modulates the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus, leading to a reduction in TP53 protein levels. In the extracellular space, it stimulates cytokine production and acts as a chemoattractant complex with PGLYRP1 to promote lymphocyte migration via CCR5 and CXCR3 receptors. The protein forms homodimers and interacts with various partners, including PPFIBP1, Annexin 2/ANXA2, TP53, CCR5, and FCGR3A, each interaction contributing to its diverse cellular functions. This intricate network of interactions underscores the multifaceted role of S100A4 in orchestrating cellular responses across different pathways. S100A4 Protein, Human (C/N-His) is the recombinant human-derived S100A4 protein, expressed by E. coli , with N-6*His, C-6*His labeled tag. The total length of S100A4 Protein, Human (C/N-His) is 101 a.a., with molecular weight of ~13 kDa.

S100A4 蛋白是一种钙结合分子，在许多细胞过程中发挥着多种作用，包括运动、血管生成、细胞分化、细胞凋亡和自噬。在功能上，它通过与非肌肉肌球蛋白重链 IIA/MYH9 相互作用，促进肌丝解聚并增加可溶性肌球蛋白-IIA 的量以促进趋化性，从而增强细胞运动性和侵袭性。此外，S100A4 通过与细胞核内的 C 端反式激活结构域结合来调节 TP53 的促凋亡功能，从而导致 TP53 蛋白水平降低。在细胞外空间，它刺激细胞因子的产生，并与 PGLYRP1 形成趋化复合物，通过 CCR5 和 CXCR3 受体促进淋巴细胞迁移。该蛋白质形成同二聚体并与各种伙伴相互作用，包括 PPFIBP1、膜联蛋白 2/ANXA2、TP53、CCR5 和 FCGR3A，每种相互作用都有助于其多样化的细胞功能。这种复杂的相互作用网络强调了 S100A4 在协调不同途径的细胞反应中的多方面作用。

Data is not available

Human

E. coli

N-6*His;C-6*His

P26447 (M1-K101)

6275  [NCBI]

MARPLEEALDVIVSTFHKYSGKEGDKFKLNKTELKELLTRELPSFLGKRTDEAAFQKVMSNLDSNRDNEVDFQEYCVFLSCIAMMCNEFFEGCPDKEPRKK

Approximately 13 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 300mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween80, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For lower concentration,please reconstitute in 50 mM Tris-HCL,300 mM NaCl, pH 7.4 buffer.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.